Human GFAP (Glial Fibrillary Acidic Protein) CLIA Kit
The Human GFAP (Glial Fibrillary Acidic Protein) CLIA Kit is a powerful tool designed for the precise measurement of GFAP levels in human samples such as serum, plasma, and cell culture supernatants. This kit is known for its exceptional sensitivity and specificity, ensuring trustworthy and consistent results, making it an excellent choice for various research applications.GFAP is a critical protein found in astrocytes, a type of glial cell in the central nervous system. GFAP is involved in providing structural support to nerve cells and is a key marker for astrocyte activation in response to various neurological conditions such as traumatic brain injury, stroke, and neurodegenerative diseases.
Understanding GFAP levels can provide valuable insights into the pathophysiology of these conditions and aid in the development of potential therapeutic strategies.Overall, the Human GFAP CLIA Kit offers researchers a reliable and efficient method for quantifying GFAP levels in human samples, making it an invaluable tool for advancing our understanding of neurological disorders and exploring novel treatment options.
Product Name:
Human GFAP (Glial Fibrillary Acidic Protein) CLIA Kit
SKU:
HUES01042
Target:
Human GFAP (Glial Fibrillary Acidic Protein)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human GFAP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GFAP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GFAP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human GFAP. You can calculate the concentration of Human GFAP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
103-120
89-103
85-96
Average (%)
110
96
91
1:4
Range (%)
97-112
101-116
98-110
Average (%)
104
107
104
1:8
Range (%)
92-106
89-102
98-111
Average (%)
100
96
105
1:16
Range (%)
99-115
97-113
87-100
Average (%)
105
104
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-106
98
EDTA plasma (n=5)
94-106
101
Cell culture media (n=5)
88-100
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
216.21
554.75
1858.1
196.81
548.78
1766.95
Standard deviation
23.24
55.25
139.73
19.62
39.9
158.67
C V (%)
10.75
9.96
7.52
9.97
7.27
8.98
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human GFAP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human GFAP in samples. No significant cross-reactivity or interference between Human GFAP and analogues was observed.
