Human General Transcription Factor IIA, Polypeptide 1 (GTF2A1) ELISA Kit
The Human General Transcription Factor IIA Polypeptide 1 (GTF2A1) ELISA Kit is a highly sensitive and specific assay designed for the precise measurement of GTF2A1 levels in human samples including serum, plasma, and cell culture supernatants. This ELISA kit provides accurate and reproducible results, making it an ideal tool for a variety of research applications.GTF2A1 is a key transcription factor involved in the regulation of gene expression and plays a crucial role in various cellular processes.
Dysregulation of GTF2A1 has been implicated in several diseases, including cancer and neurological disorders, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.With its easy-to-use format and reliable performance, the Human General Transcription Factor IIA Polypeptide 1 (GTF2A1) ELISA Kit is a valuable asset for researchers looking to study the role of GTF2A1 in health and disease.
Product Name:
Human General Transcription Factor IIA, Polypeptide 1 (GTF2A1) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
