The Human GDN (Glial-Derived Nexin) ELISA Kit is specially designed to accurately measure GDN levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results for various research purposes. GDN, also known as protease nexin-1, is a key protein involved in regulating protease activity and inhibiting blood coagulation. It plays a crucial role in various physiological processes, including inflammation, tissue remodeling, and wound healing. Dysregulation of GDN levels has been linked to conditions such as Alzheimer's disease, stroke, and inflammatory disorders, highlighting its significance as a potential biomarker for disease diagnosis and treatment.
By utilizing the Human GDN ELISA Kit, researchers can gain valuable insights into the role of GDN in health and disease, paving the way for innovative therapeutic strategies and diagnostic approaches. Its ease of use and reliable performance make it a valuable tool for studying GDN biology and its implications in various pathological conditions.
Product Name:
Human GDN (Glia Derived Nexin) ELISA Kit
SKU:
HUES03505
Target:
Human GDN (Glia Derived Nexin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
4.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human GDN. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GDN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GDN, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human GDN. You can calculate the concentration of Human GDN in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-103
99-114
95-106
Average (%)
97
106
100
1:4
Range (%)
88-101
84-96
84-98
Average (%)
95
90
90
1:8
Range (%)
92-104
82-95
84-96
Average (%)
98
89
91
1:16
Range (%)
89-103
84-95
83-96
Average (%)
96
89
89
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-101
92
EDTA plasma (n=5)
87-102
94
Cell culture media (n=5)
90-105
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.99
3.05
7.36
0.89
2.79
8.02
Standard deviation
0.06
0.12
0.27
0.04
0.13
0.43
C V (%)
6.06
3.93
3.67
4.49
4.66
5.36
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human GDN concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human GDN in samples. No significant cross-reactivity or interference between Human GDN and analogues was observed.
