Human GDI2 Recombinant Protein (RPPB3586)
- SKU:
- RPPB3586
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P50395
Description
| Product Name: | Human GDI2 Recombinant Protein |
| Product Code: | RPPB3586 |
| Size: | 10µg |
| Species: | Human |
| Target: | GDI2 |
| Synonyms: | GDP Dissociation Inhibitor 2, GDI2, RABGDIB, Guanosine Diphosphate Dissociation Inhibitor 2 , Rab GDI Beta , GDI-2, Epididymis Secretory Sperm Binding Protein Li 46e, Rab GDP Dissociation Inhibitor Beta , Rab GDP-Dissociation , HEL-S-46e. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered clear solution. |
| Formulation: | The GDI2 solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.1M NaCl, 10% glycerol and 1mM DTT. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 85.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSMNEEYDV IVLGTGLTEC ILSGIMSVNG KKVLHMDRNP YYGGESASIT PLEDLYKRFK IPGSPPESMG RGRDWNVDLI PKFLMANGQL VKMLLYTEVT RYLDFKVTEG SFVYKGGKIY KVPSTEAEAL ASSLMGLFEK RRFRKFLVYV ANFDEKDPRT FEGIDPKKTT MRDVYKKFDL GQDVIDFTGH ALALYRTDDY LDQPCYETIN RIKLYSESLA RYGKSPYLYP LYGLGELPQG FARLSAIYGG TYMLNKPIEE IIVQNGKVIG VKSEGEIARC KQLICDPSYV KDRVEKVGQV IRVICILSHP IKNTNDANSC QIIIPQNQVN RKSDIYVCMI SFAHNVAAQG KYIAIVSTTV ETKEPEKEIR PALELLEPIE QKFVSISDLL VPKDLGTESQ IFISRTYDAT THFETTCDDI KNIYKRMTGS EFDFEEMKRK KNDIYGED |
Rab GDP dissociation inhibitor beta isoform 1 (GDI2) is a member of the GDP dissociation inhibitors (GDIs) family. GDI proteins can bind and release GDP-bound Rab proteins from membranes. GDI1 interacts with virtually all of the Rab proteins, whereas GDI2 interacts with Rabll but not Rab3A. GDI2 distributes ubiquitously, presenting a membrane bound location in perinuclear regions of cells.
GDI2 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 468 amino acids (1-445) and having a molecular mass of 53.1 kDa.GDI2 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | GDI2: Regulates the GDP/GTP exchange reaction of most Rab proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them. Belongs to the Rab GDI family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:G protein regulator, misc.; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 10p15 Cellular Component: cytoplasm; cytosol; focal adhesion; vesicle Molecular Function:protein binding Biological Process: regulation of small GTPase mediated signal transduction; signal transduction |
| NCBI Summary: | GDP dissociation inhibitors are proteins that regulate the GDP-GTP exchange reaction of members of the rab family, small GTP-binding proteins of the ras superfamily, that are involved in vesicular trafficking of molecules between cellular organelles. GDIs slow the rate of dissociation of GDP from rab proteins and release GDP from membrane-bound rabs. GDI2 is ubiquitously expressed. The GDI2 gene contains many repetitive elements indicating that it may be prone to inversion/deletion rearrangements. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P50395 |
| NCBI GenInfo Identifier: | 13638228 |
| NCBI Gene ID: | 2665 |
| NCBI Accession: | P50395.2 |
| UniProt Secondary Accession: | P50395,O43928, Q5SX88, Q9UQM6, |
| UniProt Related Accession: | P50395 |
| Molecular Weight: | 45,619 Da |
| NCBI Full Name: | Rab GDP dissociation inhibitor beta |
| NCBI Synonym Full Names: | GDP dissociation inhibitor 2 |
| NCBI Official Symbol: | GDI2�� |
| NCBI Official Synonym Symbols: | RABGDIB; HEL-S-46e�� |
| NCBI Protein Information: | rab GDP dissociation inhibitor beta |
| UniProt Protein Name: | Rab GDP dissociation inhibitor beta |
| UniProt Synonym Protein Names: | Guanosine diphosphate dissociation inhibitor 2; GDI-2 |
| Protein Family: | Guanosine nucleotide diphosphate dissociation inhibitor |
| UniProt Gene Name: | GDI2�� |
| UniProt Entry Name: | GDIB_HUMAN |
