Human GDH/GLDH (Glutamate dehydrogenase) ELISA Kit
The Human GDH/GLDH (Glutamate Dehydrogenase) ELISA Kit is meticulously designed for the accurate and quantitative detection of GDH/GLDH protein levels in various human biological samples. GDH, also known as GLDH, is a key enzyme involved in amino acid metabolism and plays a crucial role in catalyzing the conversion of glutamate to α-ketoglutarate. This enzyme is vital for cellular energy production and maintaining nitrogen balance. Accurate measurement of GDH/GLDH levels using our ELISA kit can provide valuable insights into metabolic processes, hepatic function, and various pathophysiological conditions.
Our ELISA kit boasts exceptional sensitivity and specificity, ensuring precise and reproducible results. Manufactured under strict quality control standards, this kit offers reliable performance and user-friendly protocols, making it suitable for both research and clinical applications. Trust our Human GDH/GLDH ELISA Kit for robust and dependable quantification of this essential enzyme in your studies.
Product Name:
Human GDH/GLDH (Glutamate dehydrogenase) ELISA Kit
SKU:
AEES00104
Target:
Human GDH/GLDH (Glutamate dehydrogenase)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 mIU/mL
Detection range:
0.31-20 mIU/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human GDH/GLDH. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GDH/GLDH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GDH/GLDH, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human GDH/GLDH. You can calculate the concentration of Human GDH/GLDH in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-102
90-103
99-107
Average (%)
95
97
103
1:4
Range (%)
93-105
89-100
92-99
Average (%)
98
92
96
1:8
Range (%)
93-104
101-113
90-98
Average (%)
96
105
93
1:16
Range (%)
96-107
100-107
102-113
Average (%)
101
104
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-96
91
EDTA plasma (n=5)
91-101
96
Cell culture media (n=5)
83-97
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.87
2.43
8.22
0.89
2.37
8.02
Standard deviation
0.05
0.11
0.43
0.05
0.11
0.44
C V (%)
5.75
4.53
5.23
5.62
4.64
5.49
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human GDH/GLDH concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human GDH/GLDH in samples. No significant cross-reactivity or interference between Human GDH/GLDH and analogues was observed.
