Human GDF3 (Growth Differentiation Factor 3) CLIA Kit
The Human GDF3 (Growth Differentiation Factor 3) CLIA Kit is a reliable and accurate tool for measuring GDF3 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, providing consistent and precise results for various research applications.GDF3 is a key protein involved in cell growth and differentiation, playing a crucial role in various cellular processes.
Abnormal GDF3 levels have been linked to certain diseases and conditions, making it a valuable biomarker for studying and potentially developing therapies for these ailments.With the Human GDF3 CLIA Kit, researchers can gain valuable insights into the role of GDF3 in health and disease, paving the way for advancements in medical research and potential clinical applications.
Product Name:
Human GDF3 (Growth Differentiation Factor 3) CLIA Kit
SKU:
HUES01055
Target:
Human GDF3 (Growth Differentiation Factor 3)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human GDF3. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GDF3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GDF3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human GDF3. You can calculate the concentration of Human GDF3 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-97
100-115
95-110
Average (%)
90
105
101
1:4
Range (%)
87-100
104-118
100-116
Average (%)
94
110
106
1:8
Range (%)
96-106
96-111
94-108
Average (%)
101
103
99
1:16
Range (%)
89-105
91-103
87-100
Average (%)
96
96
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-108
100
EDTA plasma (n=5)
99-114
105
Cell culture media (n=5)
89-101
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
49.59
133.38
496.29
49.52
129.7
465.72
Standard deviation
5.37
12.36
53.5
5.65
13.57
40.66
C V (%)
10.83
9.27
10.78
11.41
10.46
8.73
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human GDF3 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human GDF3 in samples. No significant cross-reactivity or interference between Human GDF3 and analogues was observed.
