Human GBA3 Recombinant Protein (RPPB1678)
- SKU:
- RPPB1678
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- Q9H227
- Research Area:
- Enzymes
Description
| Product Name: | Human GBA3 Recombinant Protein |
| Product Code: | RPPB1678 |
| Size: | 20µg |
| Species: | Human |
| Target: | GBA3 |
| Synonyms: | Cytosolic beta-glucosidase, Cytosolic beta-glucosidase-like protein 1, GBA3, CBG, CBGL1, Glucosidase Beta Acid 3, GH1, Glycoside Hydrolase Family 1, GLUC, KLRP. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | GBA3 protein solution (0.25mg/ml) containing Phosphate Buffered Saline (pH7.4), 20% glycerol and 1mM DTT. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 85% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSMAFPAGF GWAAATAAYQ VEGGWDADGK GPCVWDTFTH QGGERVFKNQ TGDVACGSYT LWEEDLKCIK QLGLTHYRFS LSWSRLLPDG TTGFINQKGI DYYNKIIDDL LKNGVTPIVT LYHFDLPQTL EDQGGWLSEA IIESFDKYAQ FCFSTFGDRV KQWITINEAN VLSVMSYDLG MFPPGIPHFG TGGYQAAHNL IKAHARSWHS YDSLFRKKQK GMVSLSLFAV WLEPADPNSV SDQEAAKRAI TFHLDLFAKP IFIDGDYPEV VKSQIASMSQ KQGYPSSRLP EFTEEEKKMI KGTADFFAVQ YYTTRLIKYQ ENKKGELGIL QDAEIEFFPD PSWKNVDWIY VVPWGVCKLL KYIKDTYNNP VIYITENGFP QSDPAPLDDT QRWEYFRQTF QELFKAIQLD KVNLQVYCAW SLLDNFEWNQ GYSSRFGLFH VDFEDPARPR VPYTSAKEYA KIIRNNGLEA HL |
Glucosidase Beta Acid 3, also known as GBA3, hydrolyzes various types of glycosides. GBA3 takes part in a nonlysosomal catabolic pathway of glycosylceramide. GBA3 is a protein coding gene which acts as beta-glycosylceramidase.
GBA3 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 492 amino acids (1-469 a.a.) and having a molecular mass of 56.1kDa.GBA3 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | GBA3: Glycosidase probably involved in the intestinal absorption and metabolism of dietary flavonoid glycosides. Able to hydrolyze a broad variety of glycosides including phytoestrogens, flavonols, flavones, flavanones and cyanogens. Possesses beta- glycosylceramidase activity and may be involved in a nonlysosomal catabolic pathway of glycosylceramide. Belongs to the glycosyl hydrolase 1 family. Klotho subfamily. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Carbohydrate Metabolism - starch and sucrose; Hydrolase; EC 3.2.1.21; Other Amino Acids Metabolism - cyanoamino acid Chromosomal Location of Human Ortholog: 4p15.2 Cellular Component: cytosol Molecular Function:beta-galactosidase activity; beta-glucosidase activity; glucosylceramidase activity; glycosylceramidase activity Biological Process: glycoside catabolic process; glycosphingolipid metabolic process; glycosylceramide catabolic process |
| NCBI Summary: | The protein encoded by this gene is an enzyme that can hydrolyze several types of glycosides. This gene is a polymorphic pseudogene, with the most common allele being the functional allele that encodes the full-length protein. Some individuals, as represented by the reference genome allele, contain a single nucleotide polymorphism that results in a premature stop codon in the coding region, and therefore this allele is pseudogenic due to the failure to produce a functional full-length protein. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Mar 2013] |
| UniProt Code: | Q9H227 |
| NCBI GenInfo Identifier: | 77416427 |
| NCBI Gene ID: | 57733 |
| NCBI Accession: | Q9H227.2 |
| UniProt Secondary Accession: | Q9H227,Q32LY7, Q3MIH4, Q53GG8, Q6NSF4, Q8NHT8, Q9H3T4 Q9H4C6, |
| UniProt Related Accession: | Q9H227 |
| Molecular Weight: | 18,265 Da |
| NCBI Full Name: | Cytosolic beta-glucosidase |
| NCBI Synonym Full Names: | glucosylceramidase beta 3 (gene/pseudogene) |
| NCBI Official Symbol: | GBA3�� |
| NCBI Official Synonym Symbols: | CBG; GLUC; KLRP; CBGL1�� |
| NCBI Protein Information: | cytosolic beta-glucosidase |
| UniProt Protein Name: | Cytosolic beta-glucosidase |
| UniProt Synonym Protein Names: | Cytosolic beta-glucosidase-like protein 1 |
| Protein Family: | Cytosolic beta-glucosidase |
| UniProt Gene Name: | GBA3�� |
| UniProt Entry Name: | GBA3_HUMAN |
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