The Human GATA3 (GATA binding protein 3) CLIA Kit offered by Assay Genie is specifically designed for the precise detection of GATA3 levels in human samples, including serum, plasma, and cell culture supernatants. This advanced kit ensures exceptional sensitivity and specificity, guaranteeing accurate and consistent results for a variety of research applications.GATA3 is a critical transcription factor that plays a key role in regulating gene expression and cell differentiation. It is involved in numerous biological processes, including immune response, cell growth, and development.
Dysregulation of GATA3 has been linked to various diseases, such as cancer, allergies, and immune disorders, making it a valuable biomarker for investigating these conditions and potential therapeutic interventions.With the Human GATA3 CLIA Kit from Assay Genie, researchers can accurately measure GATA3 levels in human samples, enabling in-depth studies and advancements in the field of molecular biology and medicine. Trust Assay Genie for reliable and high-quality kits to support your research endeavors.
Product Name:
Human GATA3 (GATA Binding Protein 3) CLIA Kit
SKU:
HUES00530
Target:
Human GATA3 (GATA Binding Protein 3)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human GATA3. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GATA3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GATA3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human GATA3. You can calculate the concentration of Human GATA3 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
102-116
92-104
97-115
Average (%)
108
99
105
1:4
Range (%)
86-99
102-116
88-105
Average (%)
92
110
96
1:8
Range (%)
94-105
83-95
97-110
Average (%)
100
90
103
1:16
Range (%)
90-106
93-106
101-119
Average (%)
97
101
109
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-99
92
EDTA plasma (n=5)
95-108
102
Cell culture media (n=5)
85-99
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
185.12
568.83
1430.94
186.26
563.6
1531.82
Standard deviation
16.75
61.09
136.37
22.71
65.66
163.75
C V (%)
9.05
10.74
9.53
12.19
11.65
10.69
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human GATA3 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human GATA3 in samples. No significant cross-reactivity or interference between Human GATA3 and analogues was observed.
