Human GAP43 (Growth Associated Protein 43) CLIA Kit
The Human GAP43 (Growth-Associated Protein 43) CLIA Kit is specifically designed to accurately measure GAP43 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications. GAP43 is a protein associated with neuronal growth and regeneration, playing a crucial role in nerve development and plasticity. Its expression levels are implicated in conditions such as neural injuries, neurodegenerative diseases, and psychiatric disorders, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.
With the Human GAP43 CLIA Kit, researchers can accurately quantify GAP43 levels, allowing for a deeper understanding of its role in neuronal function and potential implications for disease pathology. Order now to elevate your research and uncover new insights into neurological conditions.
Product Name:
Human GAP43 (Growth Associated Protein 43) CLIA Kit
SKU:
HUES01061
Target:
Human GAP43 (Growth Associated Protein 43)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human GAP43. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GAP43 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GAP43, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human GAP43. You can calculate the concentration of Human GAP43 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-116
102-119
96-111
Average (%)
107
110
102
1:4
Range (%)
96-109
88-102
88-100
Average (%)
102
95
94
1:8
Range (%)
95-111
85-97
98-113
Average (%)
103
91
105
1:16
Range (%)
85-97
102-119
87-98
Average (%)
90
109
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
100-112
106
EDTA plasma (n=5)
89-101
96
Cell culture media (n=5)
93-107
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
94.41
287.04
773.36
102.98
274.69
735.86
Standard deviation
7.59
25.03
49.96
10.74
32.47
61.52
C V (%)
8.04
8.72
6.46
10.43
11.82
8.36
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human GAP43 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human GAP43 in samples. No significant cross-reactivity or interference between Human GAP43 and analogues was observed.
