Human gammaGT1 (Gamma Glutamyltransferase 1) ELISA Kit
The Human GammaGT1 (Gamma-glutamyltransferase 1) ELISA Kit is specifically designed for the accurate quantification of Gamma-glutamyltransferase 1 levels in human serum, plasma, and cell culture supernatants. This kit is highly sensitive and specific, ensuring precise and reliable results for a variety of research applications.Gamma-glutamyltransferase 1 is an important enzyme involved in the metabolism of glutathione and other peptides, playing a critical role in processes such as detoxification and antioxidant defense.
Abnormal levels of Gamma-glutamyltransferase 1 have been linked to liver diseases, kidney disorders, and other health conditions, making it a valuable biomarker for studying these diseases and potential treatment strategies.With its advanced technology and superior performance, the Human GammaGT1 ELISA Kit is an essential tool for researchers and clinicians looking to investigate the role of Gamma-glutamyltransferase 1 in various health conditions and therapeutic interventions.
Product Name:
Human gammaGT1 (Gamma Glutamyltransferase 1) ELISA Kit
SKU:
HUES02119
Target:
Human gammaGT1 (Gamma Glutamyltransferase 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human γGT1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human γGT1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human γGT1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human γGT1. You can calculate the concentration of Human γGT1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-103
87-103
88-100
Average (%)
95
94
94
1:4
Range (%)
87-99
87-100
90-100
Average (%)
94
92
95
1:8
Range (%)
88-101
86-100
83-99
Average (%)
93
92
90
1:16
Range (%)
90-102
85-99
84-98
Average (%)
95
91
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-110
100
EDTA plasma (n=5)
89-102
95
Cell culture media (n=5)
91-104
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
4.64
11.93
39.33
4.9
12.5
40.75
Standard deviation
0.27
0.66
1.38
0.3
0.63
2.01
C V (%)
5.82
5.53
3.51
6.12
5.04
4.93
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human γGT1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human γGT1 in samples. No significant cross-reactivity or interference between Human γGT1 and analogues was observed.
