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Human Gamma-Glutamyltransferase 1 (gGT1) ELISA Kit

SKU:
HUDL01179
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P19440
Sensitivity:
0.59ng/mL
Range:
1.56-100ng/mL
ELISA Type:
Sandwich
Synonyms:
CD224,G-GT1,GTG,Gamma-glutamyltranspeptidase 1,Glutathione hydrolase 1,Leukotriene-C4 hydrolase
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€649
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Description

system_update_altDatasheet

Human Gamma-Glutamyltransferase 1 (gGT1) ELISA Kit

The Human Gamma-Glutamyltransferase-1 (GGT1) ELISA Kit is a reliable and accurate tool for measuring levels of GGT1 in human samples including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, providing consistent and reproducible results for various research applications.GGT1 is an important enzyme involved in the metabolism of glutathione, playing a critical role in the detoxification process within the body.

Abnormal levels of GGT1 have been associated with liver diseases, alcohol consumption, and other health conditions, making it a valuable biomarker for studying these diseases and potential therapeutic interventions.Overall, the Human GGT1 ELISA Kit is a valuable resource for researchers and healthcare professionals looking to accurately measure and study GGT1 levels in human samples.

Product Name:Human Gamma-Glutamyltransferase 1 (gGT1) ELISA Kit
Product Code:HUDL01179
Size:96 Assays
Target:gGT1
Synonyms:CD224, G-GT1, GTG, Gamma-glutamyltranspeptidase 1, Glutathione hydrolase 1, Leukotriene-C4 hydrolase
Detection Method:Sandwich
Range:1.56-100ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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