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Human Galectin 3 (GAL3) ELISA Kit

SKU:
HUDL01143
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P17931
Sensitivity:
0.057ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
LGALS3,CBP35,GALBP,GALIG,MAC2,35 kDa lectin,Carbohydrate-binding protein 35,Galactose-specific lectin 3,Galactoside-binding protein,Laminin-binding protein,Lectin L-29
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€649
Frequently bought together:

Description

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Human Galectin 3 (GAL3) ELISA Kit

The Human Galectin-3 (Gal-3) ELISA Kit is a powerful tool for accurately measuring galectin-3 levels in human biological samples such as serum, plasma, and cell culture supernatants. With outstanding sensitivity and specificity, this kit delivers consistent and trustworthy results, making it a valuable resource for a wide range of research studies.Galectin-3 is a multifunctional protein involved in numerous biological processes, including cell adhesion, migration, and inflammation. Its dysregulation has been linked to various diseases such as cancer, fibrosis, and heart failure, highlighting its importance as a potential diagnostic and therapeutic target.

By utilizing the Human Galectin-3 (Gal-3) ELISA Kit, researchers can uncover valuable insights into the role of galectin-3 in disease pathogenesis and progression, aiding in the development of novel treatment strategies. Trust in this reliable assay to enhance your research and advance scientific knowledge in the field of galectin-3 biology.

Product Name:Human Galectin 3 (GAL3) ELISA Kit
Product Code:HUDL01143
Size:96 Assays
Target:GAL3
Synonyms:LGALS3, CBP35, GALBP, GALIG, MAC2, 35 kDa lectin, Carbohydrate-binding protein 35, Galactose-specific lectin 3, Galactoside-binding protein, Laminin-binding protein, Lectin L-29
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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