The Human Gal9 (Galectin-9) ELISA Kit is specifically designed for the precise measurement of Gal9 levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results, making it perfect for various research purposes.Gal9 is a key protein that plays a vital role in immune regulation and inflammation. It is involved in various diseases such as cancer, autoimmune disorders, and infectious diseases, making it a valuable biomarker for studying these conditions and developing therapeutic interventions.
With its user-friendly protocol and reliable performance, the Human Gal9 ELISA Kit is a valuable tool for researchers working in fields such as immunology, oncology, and infectious diseases. Don't miss the opportunity to enhance your research with this cutting-edge kit.
Product Name:
Human GAL9 (Galectin 9 ) ELISA Kit
SKU:
HUES02158
Target:
Human GAL9 (Galectin 9 )
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human GAL9. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GAL9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GAL9, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human GAL9. You can calculate the concentration of Human GAL9 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-103
86-96
94-111
Average (%)
96
91
101
1:4
Range (%)
92-105
81-91
82-93
Average (%)
98
86
87
1:8
Range (%)
92-107
82-92
89-101
Average (%)
98
86
94
1:16
Range (%)
89-102
80-93
86-97
Average (%)
95
87
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-108
99
EDTA plasma (n=5)
83-96
90
Cell culture media (n=5)
92-105
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
22.95
70.96
204.84
24.81
71.23
194.4
Standard deviation
1.32
3.73
6.21
1.6
3.4
8.16
C V (%)
5.75
5.26
3.03
6.45
4.77
4.2
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human GAL9 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human GAL9 in samples. No significant cross-reactivity or interference between Human GAL9 and analogues was observed.
Shete et al.
Dynamics of Matricellular Protein Levels in Blood Predict Recovery in Patients with Human Immunodeficiency Virus-Tuberculosis Coinfection
