The Human Gal9 (Galectin-9) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of Gal9 levels in human serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it an ideal tool for researchers investigating the role of Gal9 in various physiological and pathological processes.Gal9 is a multifunctional protein that plays a critical role in regulating immune responses, inflammation, and cell growth. Dysregulation of Gal9 expression has been linked to various diseases, including cancer, autoimmune disorders, and infectious diseases.
As such, measuring Gal9 levels can provide valuable insights into disease mechanisms and potential therapeutic targets.The Human Gal9 CLIA Kit from Assay Genie offers researchers a convenient and reliable method for quantifying Gal9 levels in biological samples, facilitating the study of Gal9's role in health and disease. With its high sensitivity and specificity, this kit is a valuable tool for advancing research in immunology, oncology, and other fields.
Product Name:
Human GAL9 (Galectin 9 ) CLIA Kit
SKU:
HUES00624
Target:
Human GAL9 (Galectin 9 )
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human GAL9. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GAL9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GAL9, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human GAL9. You can calculate the concentration of Human GAL9 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-102
102-118
102-121
Average (%)
97
108
110
1:4
Range (%)
83-96
97-112
92-102
Average (%)
90
103
97
1:8
Range (%)
99-113
86-97
97-111
Average (%)
105
91
103
1:16
Range (%)
95-110
95-108
98-114
Average (%)
101
102
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-100
91
EDTA plasma (n=5)
89-101
94
Cell culture media (n=5)
95-109
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
27.74
78.32
183.16
25.84
82.5
170.61
Standard deviation
3.37
8.38
17.25
2.8
9.31
19.48
C V (%)
12.15
10.7
9.42
10.84
11.28
11.42
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human GAL9 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human GAL9 in samples. No significant cross-reactivity or interference between Human GAL9 and analogues was observed.
