The Human Galectin-14 (Gal-14) ELISA Kit is specifically designed for the accurate quantification of Galectin-14 levels in human biological samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and reproducible results for a wide range of research applications.Galectin-14 is a member of the galectin family of proteins that play a crucial role in various biological processes, including cell adhesion, proliferation, and apoptosis.
Galectin-14 has been implicated in cancer progression, inflammation, and immune response regulation, making it a valuable biomarker for studying these conditions and potentially developing therapeutic interventions.Overall, the Human Galectin-14 (Gal-14) ELISA Kit provides researchers with a powerful tool for investigating the role of Galectin-14 in health and disease, offering reliable and accurate measurements for meaningful scientific discoveries and advancements.
Product Name:
Human Galectin 14 (GAL14) ELISA Kit
Product Code:
HUDL01140
Size:
96 Assays
Target:
GAL14
Synonyms:
LGALS14, CLC2, PPL13, Placental Protein 13-Like, Lectin,Galactoside-Binding Soluble 14, Charcot-Leyden crystal protein 2
Detection Method:
Sandwich
Range:
0.312-20 ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-103
96
Heparin plasma (n=5)
89-102
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
