The Human Gal14 (Galectin-14) CLIA Kit is a powerful tool for measuring Gal14 levels in human biological samples such as serum, plasma, and cell culture supernatants. This kit is specifically designed for high sensitivity and specificity, ensuring accurate and reliable results for a variety of research applications.Galectin-14 is a protein known to play a critical role in various biological processes, including cell adhesion, cell growth, and immune responses.
Dysregulation of Galectin-14 has been associated with inflammatory diseases, cancer, and autoimmune disorders, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.With its advanced technology and user-friendly design, the Human Gal14 CLIA Kit is a valuable asset for researchers looking to delve deeper into the role of Galectin-14 in human health and disease.
Product Name:
Human GAL14 (Galectin 14 ) CLIA Kit
SKU:
HUES00618
Target:
Human GAL14 (Galectin 14 )
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human GAL14. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GAL14 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GAL14, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human GAL14. You can calculate the concentration of Human GAL14 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-112
103-115
97-110
Average (%)
103
109
103
1:4
Range (%)
97-114
94-110
103-119
Average (%)
105
101
110
1:8
Range (%)
91-107
99-112
87-103
Average (%)
97
106
94
1:16
Range (%)
87-98
87-100
98-115
Average (%)
92
94
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
101-114
107
EDTA plasma (n=5)
95-108
102
Cell culture media (n=5)
94-106
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
209.77
545.47
1838.52
209.59
589.67
1849.92
Standard deviation
24.67
40.8
174.84
23.6
64.04
124.13
C V (%)
11.76
7.48
9.51
11.26
10.86
6.71
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human GAL14 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human GAL14 in samples. No significant cross-reactivity or interference between Human GAL14 and analogues was observed.
