Human GAD1 Recombinant Protein (RPPB1663)
- SKU:
- RPPB1663
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- Q99259
- Research Area:
- Enzymes
Description
| Product Name: | Human GAD1 Recombinant Protein |
| Product Code: | RPPB1663 |
| Size: | 10µg |
| Species: | Human |
| Target: | GAD1 |
| Synonyms: | Glutamate Decarboxylase 1 (Brain, 67kDa), GAD, Glutamate Decarboxylase 67 KDa Isoform, 67 KDa Glutamic Acid Decarboxylase, GAD-67, EC 4.1.1.15, CPSQ1, SCP, Glutamate Decarboxylase 1 (Brain, 67kD), Glutamate Decarboxylase 1, GAD67, EC 4.1.1, GAD1. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The GAD1 solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH8.0), 10% glycerol and 0.4M Urea. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 85% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSMASSTPS SSATSSNAGA DPNTTNLRPT TYDTWCGVAH GCTRKLGLKI CGFLQRTNSL EEKSRLVSAF KERQSSKNLL SCENSDRDAR FRRTETDFSN LFARDLLPAK NGEEQTVQFL LEVVDILLNY VRKTFDRSTK VLDFHHPHQL LEGMEGFNLE LSDHPESLEQ ILVDCRDTLK YGVRTGHPRF FNQLSTGLDI IGLAGEWLTS TANTNMPSDM RECWLLR |
Glutamate Decarboxylase 1 (GAD1) is one of several forms of glutamic acid decarboxylase. GAD1 is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for the GAD1 enzyme has been identified in the human pancreas since it has been detected as an autoantigen and an autoreactive T cell target type II diabetes. The GAD1 protein may also have a role in the stiff man syndrome. GAD1 enzyme deficiency leads to pyridoxine dependency with seizures. GAD1 also catalyzes the production of GABA.
GAD1 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 247 amino acids (1-224) and having a molecular mass of 27.7kDa.GAD1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | GAD67: Catalyzes the production of GABA. Homodimer. Isoform 3 is expressed in pancreatic islets, testis, adrenal cortex, and perhaps other endocrine tissues, but not in brain. Belongs to the group II decarboxylase family. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:EC 4.1.1.15; Other Amino Acids Metabolism - beta-alanine; Other Amino Acids Metabolism - taurine and hypotaurine; Carbohydrate Metabolism - butanoate; Amino Acid Metabolism - alanine, aspartate and glutamate; Mitochondrial; Lyase Chromosomal Location of Human Ortholog: 2q31 Cellular Component: plasma membrane; intracellular; vesicle membrane Molecular Function:protein binding; glutamate binding; glutamate decarboxylase activity; protein heterodimerization activity; protein N-terminus binding; pyridoxal phosphate binding Biological Process: response to drug; glutamate catabolic process; synaptic transmission; glutamate decarboxylation to succinate; neurotransmitter secretion; gamma-aminobutyric acid biosynthetic process; protein-pyridoxal-5-phosphate linkage; neurotransmitter biosynthetic process Disease: Cerebral Palsy, Spastic Quadriplegic, 1 |
| NCBI Summary: | This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Deficiency in this enzyme has been shown to lead to pyridoxine dependency with seizures. Alternative splicing of this gene results in two products, the predominant 67-kD form and a less-frequent 25-kD form. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q99259 |
| NCBI GenInfo Identifier: | 1352213 |
| NCBI Gene ID: | 2571 |
| NCBI Accession: | Q99259.1 |
| UniProt Secondary Accession: | Q99259,Q49AK1, Q53TQ7, Q9BU91, Q9UHH4, |
| UniProt Related Accession: | Q99259 |
| Molecular Weight: | 66,897 Da |
| NCBI Full Name: | Glutamate decarboxylase 1 |
| NCBI Synonym Full Names: | glutamate decarboxylase 1 (brain, 67kDa) |
| NCBI Official Symbol: | GAD1�� |
| NCBI Official Synonym Symbols: | GAD; SCP; CPSQ1�� |
| NCBI Protein Information: | glutamate decarboxylase 1; GAD-67; 67 kDa glutamic acid decarboxylase; glutamate decarboxylase 67 kDa isoform |
| UniProt Protein Name: | Glutamate decarboxylase 1 |
| UniProt Synonym Protein Names: | 67 kDa glutamic acid decarboxylase; GAD-67; Glutamate decarboxylase 67 kDa isoform |
| Protein Family: | Glutamate decarboxylase |
| UniProt Gene Name: | GAD1�� |
| UniProt Entry Name: | DCE1_HUMAN |
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