Human GABARAP Recombinant Protein (RPPB3567)
- SKU:
- RPPB3567
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- O95166
Description
| Product Name: | Human GABARAP Recombinant Protein |
| Product Code: | RPPB3567 |
| Size: | 50µg |
| Species: | Human |
| Target: | GABARAP |
| Synonyms: | GBRAP, MM46, GABA(A) receptor-associated protein, Gamma-aminobutyric acid receptor-associated protein, GABARAP, FLJ25768, MGC120154, MGC120155, FLC3B. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | GABARAP solution (1mg/ml)�containing 20mM Tris-HCl pH-8, 0.2M NaCl and 20% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MKFVYKEEHP FEKRRSEGEK IRKKYPDRVP VIVEKAPKAR IGDLDKKKYL VPSDLTVGQF YFLIRKRIHL RAEDALFFFV NNVIPPTSAT MGQLYQEHHE EDFFLYIAYS DESVYGL |
GABARAP is a ligand-gated chloride channel that mediates inhibitory neurotransmission. GABARAP is highly positively charged in its N-terminus and shares sequence similarity with light chain-3 of microtubule-associated proteins 1A and 1B. GABARAP clusters neurotransmitter receptors by mediating interaction with the cytoskeleton.
GABARAP Human Recombinant produced in E.Coli is a single, non-glycosylated,polypeptide chain containing 137 amino acids (1-117 a.a.) and having a molecular mass of 16 kDa. GABARAP is fused to a 20 amino acid His-Tag at N-Terminus and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | GABARAP: is a ubiquitin-like protein that is a constituent of the ATG8-conjugation system, one of two evolutionarily conserved phosphatidylethanolamine conjugation systems necessary for the formation of the autophagosome. The human ATG8 system includes seven ubiquitin-like light chain proteins (LCPs) that are homologs of yeast LC3: MAP1LC3A, -B, -C, GABARAP, GABARAPL1, -2, and -3. Pro-LCPs are cleaved by ATG4B to expose a C-terminal glycine residue, the cytosolic LCP-I form. The exposed C-terminus is conjugated to the head group amine of phosphatidylethanolamine (PE) through an amide bond by a sequence of ubiquitination-like reactions that involves an E1 (ATG7), an E2 (ATG3), and an E3 (a complex including ATG5, ATG12, and ATG16L). The PE-congugated form (LCP-II) is tightly associated with the autophagosomal membrane. The LCP-II forms can also be delipidated by the ATG4 proteases: most of the LCPs are delipidated and liberated from the membrane before autophagosomes fuse with lysosomes. May play a role in intracellular transport of GABA(A) receptors and its interaction with the cytoskeleton. Belongs to the MAP1 LC3 family. Interacts with GPHN and NSF. Interacts with GABRG2, beta-tubulin and ULK1. |
| UniProt Protein Details: | Protein type:Autophagy; Adaptor/scaffold; Microtubule-binding; Ubiquitin-like modifier; Vesicle Chromosomal Location of Human Ortholog: 17p13.1 Cellular Component: microtubule; smooth endoplasmic reticulum; lysosome; autophagic vacuole; cytosol; actin cytoskeleton; Golgi membrane; microtubule associated complex; extrinsic to membrane; perinuclear region of cytoplasm; plasma membrane; axoneme; cytoplasmic vesicle Molecular Function:protein binding; microtubule binding; GABA receptor binding; beta-tubulin binding Biological Process: synaptic transmission; induction of apoptosis via death domain receptors; mitochondrion degradation; microtubule cytoskeleton organization and biogenesis; autophagic vacuole formation; protein targeting |
| NCBI Summary: | Gamma-aminobutyric acid A receptors [GABA(A) receptors] are ligand-gated chloride channels that mediate inhibitory neurotransmission. This gene encodes GABA(A) receptor-associated protein, which is highly positively charged in its N-terminus and shares sequence similarity with light chain-3 of microtubule-associated proteins 1A and 1B. This protein clusters neurotransmitter receptors by mediating interaction with the cytoskeleton. [provided by RefSeq, Jul 2008] |
| UniProt Code: | O95166 |
| NCBI GenInfo Identifier: | 44887916 |
| NCBI Gene ID: | 11337 |
| NCBI Accession: | O95166.1 |
| UniProt Related Accession: | O95166 |
| Molecular Weight: | 117 |
| NCBI Full Name: | Gamma-aminobutyric acid receptor-associated protein |
| NCBI Synonym Full Names: | GABA(A) receptor-associated protein |
| NCBI Official Symbol: | GABARAP�� |
| NCBI Official Synonym Symbols: | MM46; ATG8A; GABARAP-a�� |
| NCBI Protein Information: | gamma-aminobutyric acid receptor-associated protein |
| UniProt Protein Name: | Gamma-aminobutyric acid receptor-associated protein |
| UniProt Synonym Protein Names: | GABA(A) receptor-associated protein; MM46 |
| Protein Family: | Gamma-aminobutyric acid receptor-associated protein |
| UniProt Gene Name: | GABARAP�� |
| UniProt Entry Name: | GBRAP_HUMAN |
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