null

Human GA Binding Protein Transcription Factor Alpha (GABPa) ELISA Kit

SKU:
HUDL01133
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q06546
Sensitivity:
0.057ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
E4TF1-60,E4TF1A,NFT2,NRF2,NRF2A,Nuclear Respiratory Factor-2 Subunit Alpha,Transcription factor E4TF1-60
Reactivity:
Human
Sample Type:
Tissue Homogenates And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Human GA Binding Protein Transcription Factor Alpha (GABPa) ELISA Kit

The Human GA Binding Protein Transcription Factor Alpha (GABPA) ELISA Kit is specifically designed for the precise measurement of GABPA levels in human samples such as serum, plasma, and cell culture supernatants. With exceptional sensitivity and specificity, this kit delivers accurate and consistent results, making it an invaluable tool for various research applications.GABPA is a pivotal transcription factor involved in regulating gene expression and plays a vital role in various cellular processes, including cell proliferation, differentiation, and apoptosis.

Dysregulation of GABPA has been linked to several diseases, including cancer and metabolic disorders, highlighting its significance as a potential biomarker for disease diagnosis and therapeutic development.Invest in the Human GABPA ELISA Kit to advance your research and gain valuable insights into the role of GABPA in health and disease.

Product Name:Human GA Binding Protein Transcription Factor Alpha (GABPa) ELISA Kit
Product Code:HUDL01133
Size:96 Assays
Target:GABPa
Synonyms:E4TF1-60, E4TF1A, NFT2, NRF2, NRF2A, Nuclear Respiratory Factor-2 Subunit Alpha, Transcription factor E4TF1-60
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

Related Products