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Human G Protein Coupled Receptor, Family C, Group 5, Member A (GPRC5A) ELISA Kit

SKU:
HUDL01244
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q8NFJ5
Sensitivity:
0.116ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
RAIG1,RAI3, GPCR5A,Retinoic Acid Induced 3,Orphan G-protein-coupling receptor PEIG-1,Retinoic acid-induced gene 1 protein
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€749
Frequently bought together:

Description

system_update_altDatasheet

Human G Protein Coupled Receptor, Family C, Group 5, Member A (GPRC5A) ELISA Kit

The Human G Protein-Coupled Receptor Family C Group 5 Member A (GPRC5A) ELISA Kit is specifically designed for the precise detection of GPRC5A levels in human serum, plasma, and cell culture supernatants. This advanced kit offers unmatched sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.GPRC5A is a key member of the G protein-coupled receptor family, playing a critical role in signal transduction and cell communication. Abnormal expression of GPRC5A has been linked to various diseases, including cancer, cardiovascular disorders, and neurological conditions.

Therefore, this ELISA kit serves as a valuable tool for investigating these diseases and developing potential therapeutic interventions.Overall, the Human GPRC5A ELISA Kit provides researchers with a reliable and efficient means of quantifying GPRC5A levels, facilitating in-depth studies on its biological functions and pathological implications.

Product Name:Human G Protein Coupled Receptor, Family C, Group 5, Member A (GPRC5A) ELISA Kit
Product Code:HUDL01244
Size:96 Assays
Target:GPRC5A
Synonyms:RAIG1, RAI3, GPCR5A, Retinoic Acid Induced 3, Orphan G-protein-coupling receptor PEIG-1, Retinoic acid-induced gene 1 protein
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)90-9994
Heparin plasma (n=5)91-10196
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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