Human G CSF Recombinant Protein (RPPB0318)
- SKU:
- RPPB0318
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P09919
- Research Area:
- Growth Factors & Cytokines
Description
| Product Name: | Human G CSF Recombinant Protein |
| Product Code: | RPPB0318 |
| Size: | 10µg |
| Species: | Human |
| Target: | G CSF |
| Synonyms: | CSF-3, MGI-1G, GM-CSF beta, Pluripoietin, Filgrastim, Lenograstim, G-CSF, MGC45931, GCSF. |
| Source: | Chinese Hamster Ovary Cells (CHO) |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | G-CSF was lyophilized from a concentrated (1mg/ml) solution containing 10mM Hydrochloric Acid pH=6.5, 0.4mg tween 20, 100mg mannitol, 160mg L-arginine, 40mg phenylalanine and 4mg methionine. |
| Solubility: | It is recommended to reconstitute the lyophilized Granulocyte Colony Stimulating Factor in sterile 18M?-cm H2O not less than 100�g/ml, which can then be further diluted to other aqueous solutions. |
| Stability: | Lyophilized Granulocyte Colony Stimulating Factor although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution G-CSF should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
| Purity: | Greater than 97.0% as determined by�SDS-PAGE. |
| Amino Acid Sequence: | TPLGPASSLP QSFLLKCLEQ VRKIQGDGAA LQEKLCATYK LCHPEELVLL GHSLGIPWAP LSSCPSQALQ LAGCLSQLHS GLFLYQGLLQ ALEGISPELG PTLDTLQLDV ADFATTIWQQ MEELGMAPAL QPTQGAMPAF ASAFQRRAGG VLVASHLQSF LEVSYRVLRH LAQP |
| Biological Activity: | The ED50, calculated by the dose-dependant proliferation of murine NFS-60 indicator cells (measured by 3H-thymidine uptake) is < 0.07 ng/ml, corresponding to a Specific Activity of 1.27 x 108 IU/mg. |
Granulocyte Colony Stimulating Factor is a growth factor and/or cytokine produced by the endothelium, macrophages and a number of other immune cells. GCSF stimulates the bone marrow to produce granulocytes and also to stimulate the survival, proliferation, differentiation and function of neutrophil granulocyte progenator cells and mature neutrophils.
Granulocyte Colony Stimulating Factor Human Recombinant produced in CHO cells�is a single, glycosylated, polypeptide chain containing 174 amino acids and having a molecular mass of approximately 18 kDa.G-CSF is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | G-CSF: Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes. Belongs to the IL-6 superfamily. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Cytokine; Secreted, signal peptide; Cell cycle regulation; Secreted Chromosomal Location of Human Ortholog: 17q11.2-q12 Cellular Component: extracellular space Molecular Function:enzyme binding; growth factor activity; cytokine activity; granulocyte colony-stimulating factor receptor binding Biological Process: positive regulation of protein binding; positive regulation of myeloid cell differentiation; granulocyte differentiation; multicellular organismal development; cytokine and chemokine mediated signaling pathway; positive regulation of peptidyl-serine phosphorylation; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein kinase B signaling cascade; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of actin filament polymerization; positive regulation of cell proliferation; positive regulation of transcription factor import into nucleus; immune response; positive regulation of transcription from RNA polymerase II promoter |
| NCBI Summary: | The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes. The active protein is found extracellularly. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, May 2010] |
| UniProt Code: | P09919 |
| NCBI GenInfo Identifier: | 117564 |
| NCBI Gene ID: | 1440 |
| NCBI Accession: | P09919.1 |
| UniProt Secondary Accession: | P09919,A8MXR7, |
| UniProt Related Accession: | P09919 |
| Molecular Weight: | 18,434 Da |
| NCBI Full Name: | Granulocyte colony-stimulating factor |
| NCBI Synonym Full Names: | colony stimulating factor 3 (granulocyte) |
| NCBI Official Symbol: | CSF3�� |
| NCBI Official Synonym Symbols: | GCSF; CSF3OS; C17orf33�� |
| NCBI Protein Information: | granulocyte colony-stimulating factor; filgrastim; lenograstim; pluripoietin |
| UniProt Protein Name: | Granulocyte colony-stimulating factor |
| UniProt Synonym Protein Names: | Pluripoietin; INN: Filgrastim; INN: Lenograstim |
| Protein Family: | Granulocyte colony-stimulating factor |
| UniProt Gene Name: | CSF3�� |
| UniProt Entry Name: | CSF3_HUMAN |
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