Human G-CSF (Granulocyte Colony Stimulating Factor 3) ELISA Kit
The Human G-CSF (Granulocyte Colony Stimulating Factor) ELISA Kit is specially designed for precise and accurate measurement of G-CSF levels in human serum, plasma, and cell culture supernatants. This ELISA kit offers exceptional sensitivity and specificity, ensuring consistent and reliable results for various research applications.G-CSF is a vital protein that plays a key role in stimulating the production and maturation of granulocytes, a type of white blood cell essential for immune response. Abnormal levels of G-CSF have been associated with various medical conditions, including leukemia, autoimmune diseases, and infections.
Therefore, measuring G-CSF levels can provide valuable insights into these diseases and potential treatment strategies.With its advanced technology and reliable performance, the Human G-CSF ELISA Kit is an indispensable tool for researchers and clinicians studying the role of G-CSF in health and disease. It offers a convenient and efficient method for quantifying G-CSF levels, facilitating groundbreaking discoveries and advancements in the field of biomedicine.
Product Name:
Human G-CSF (Granulocyte Colony Stimulating Factor 3) ELISA Kit
SKU:
HUES01366
Target:
Human G-CSF (Granulocyte Colony Stimulating Factor 3)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
23.44 pg/mL
Detection range:
39.06-2500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human G-CSF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human G-CSF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human G-CSF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human G-CSF. You can calculate the concentration of Human G-CSF in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-104
94-108
88-101
Average (%)
97
100
94
1:4
Range (%)
92-110
81-92
98-114
Average (%)
100
87
104
1:8
Range (%)
99-114
86-100
90-103
Average (%)
106
92
97
1:16
Range (%)
93-109
81-95
95-111
Average (%)
101
87
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-98
93
EDTA plasma (n=5)
86-98
93
Cell culture media (n=5)
84-100
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
124.9
276.5
1226.6
114.7
303.5
1183.6
Standard deviation
7.2
11.9
66.2
6.2
16.4
60.4
C V (%)
5.76
4.3
5.4
5.41
5.4
5.1
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human G-CSF concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human G-CSF in samples. No significant cross-reactivity or interference between Human G-CSF and analogues was observed.
