The Human Fucosyltransferase 3 (FUT3) ELISA Kit is a reliable and accurate tool for measuring FUT3 levels in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides consistent and reproducible results, making it an essential asset for researchers in various fields.FUT3 is an enzyme responsible for the transfer of fucose molecules to glycoproteins and glycolipids, playing a crucial role in various biological processes such as cell-cell interactions, immune responses, and disease progression.
Abnormal FUT3 expression has been linked to conditions like cancer, inflammatory diseases, and congenital disorders, making it a valuable biomarker for studying these diseases and developing targeted therapies.By using the Human FUT3 ELISA Kit, researchers can accurately quantify FUT3 levels in human samples, allowing for a better understanding of its role in disease pathogenesis and potentially leading to the development of novel diagnostic and treatment strategies.
Product Name:
Human Fucosyltransferase 3 (FUT3) ELISA Kit
Product Code:
HUDL01123
Size:
96 Assays
Target:
FUT3
Synonyms:
CD174, FUT3, FT3B, LE, Les, FucT-III, Lewis FT, Galactoside 3(4)-L-Fucosyltransferase, Lewis Blood Group, Blood group Lewis alpha-4-fucosyltransferase
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
