Human FUBP1 Recombinant Protein (RPPB3561)
- SKU:
- RPPB3561
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- Q96AE4
Description
| Product Name: | Human FUBP1 Recombinant Protein |
| Product Code: | RPPB3561 |
| Size: | 20µg |
| Species: | Human |
| Target: | FUBP1 |
| Synonyms: | Far upstream element (FUSE) binding protein 1, FUSE-binding protein 1, DNA helicase V, FUBP, FBP, hDH V. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The FUBP1 solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 1mM DTT, 0.15M NaCl and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 85% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSHMDVPIP RFAVGIVIGR NGEMIKKIQN DAGVRIQFKP DDGTTPERIA QITGPPDRCQ HAAEIITDLL RSVQAGNPGG PGPGGRGRGR GQGNWNMGPP GGLQEFNFIV PTGKTGLIIG KGGETIKSIS QQSGARIELQ RNPPPNADPN MKLFTIRGTP QQIDYARQLI EEKIG |
FUBP1 is a ssDNA binding protein which stimulates expression of c-myc in undifferentiated cells and activates the far upstream element (FUSE) of c-myc. Regulation of FUSE by FUBP happens by single-strand binding of FUBP to the non-coding strand. FUBP1 performs as an ATP-dependent DNA helicase.
FUBP1 Human Recombinant produced in E. coli is a single polypeptide chain containing 195 amino acids (279-448) and having a molecular mass of 20.8 kDa.FUBP1 is fused to a 25 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | FBP1: Regulates MYC expression by binding to a single-stranded far-upstream element (FUSE) upstream of the MYC promoter. May act both as activator and repressor of transcription. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:DNA-binding; Transcription factor; RNA-binding; Motility/polarity/chemotaxis; RNA splicing Chromosomal Location of Human Ortholog: 1p31.1 Cellular Component: nucleoplasm; nucleus Molecular Function:protein binding; transcription factor activity; single-stranded DNA binding Biological Process: transcription from RNA polymerase II promoter; positive regulation of transcription from RNA polymerase II promoter |
| NCBI Summary: | The protein encoded by this gene is a single stranded DNA-binding protein that binds to multiple DNA elements, including the far upstream element (FUSE) located upstream of c-myc. Binding to FUSE occurs on the non-coding strand, and is important to the regulation of c-myc in undifferentiated cells. This protein contains three domains, an amphipathic helix N-terminal domain, a DNA-binding central domain, and a C-terminal transactivation domain that contains three tyrosine-rich motifs. The N-terminal domain is thought to repress the activity of the C-terminal domain. This protein is also thought to bind RNA, and contains 3'-5' helicase activity with in vitro activity on both DNA-DNA and RNA-RNA duplexes. Aberrant expression of this gene has been found in malignant tissues, and this gene is important to neural system and lung development. Binding of this protein to viral RNA is thought to play a role in several viral diseases, including hepatitis C and hand, foot and mouth disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014] |
| UniProt Code: | Q96AE4 |
| NCBI GenInfo Identifier: | 116241370 |
| NCBI Gene ID: | 8880 |
| NCBI Accession: | Q96AE4.3 |
| UniProt Secondary Accession: | Q96AE4,Q12828, |
| UniProt Related Accession: | Q96AE4 |
| Molecular Weight: | 653 |
| NCBI Full Name: | Far upstream element-binding protein 1 |
| NCBI Synonym Full Names: | far upstream element (FUSE) binding protein 1 |
| NCBI Official Symbol: | FUBP1�� |
| NCBI Official Synonym Symbols: | FBP; FUBP; hDH V�� |
| NCBI Protein Information: | far upstream element-binding protein 1; DNA helicase V |
| UniProt Protein Name: | Far upstream element-binding protein 1 |
| UniProt Synonym Protein Names: | DNA helicase V; hDH V |
| Protein Family: | Far upstream element-binding protein |
| UniProt Gene Name: | FUBP1�� |
| UniProt Entry Name: | FUBP1_HUMAN |
