The Human FTL (Ferritin Light Polypeptide) CLIA Kit is a highly sensitive and specific assay designed for the precise measurement of ferritin levels in human samples such as serum and plasma. This kit is ideal for research applications where accurate quantification of ferritin is crucial.Ferritin is a key protein involved in iron storage and regulation, playing a vital role in various physiological processes. Abnormal levels of ferritin have been associated with conditions such as iron deficiency anemia, hemochromatosis, and inflammatory disorders, making it a valuable biomarker for studying these diseases and developing therapeutic interventions.
With its reliable performance and robust analytical capabilities, the Human FTL CLIA Kit from Assay Genie is an invaluable tool for researchers and clinicians alike seeking to explore the role of ferritin in health and disease.
Product Name:
Human FTL (Ferritin, Light Polypeptide) CLIA Kit
SKU:
HUES01086
Target:
Human FTL (Ferritin, Light Polypeptide)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.38 ng/mL
Detection range:
0.63-40 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human FTL. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FTL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FTL, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human FTL. You can calculate the concentration of Human FTL in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-117
94-111
100-114
Average (%)
107
102
108
1:4
Range (%)
93-106
101-118
83-97
Average (%)
99
108
90
1:8
Range (%)
93-103
87-102
94-106
Average (%)
98
94
99
1:16
Range (%)
86-98
101-119
88-100
Average (%)
91
109
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-99
93
EDTA plasma (n=5)
91-106
98
Cell culture media (n=5)
98-109
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.07
3.4
18.21
2.15
3.08
16.94
Standard deviation
0.24
0.4
1.67
0.2
0.23
1.55
C V (%)
11.59
11.76
9.17
9.3
7.47
9.15
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human FTL concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human FTL in samples. No significant cross-reactivity or interference between Human FTL and analogues was observed.
