Human FSTL1 (Follistatin Like Protein 1) ELISA Kit
The Human FSTL1 (Follistatin-Like Protein 1) ELISA Kit is specifically designed for the precise measurement of FSTL1 levels in human serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit delivers accurate and consistent results, making it an invaluable tool for various research applications.FSTL1 is a critical protein involved in multiple biological processes, including inflammation, tissue repair, and cell proliferation. It also plays a crucial role in cardiovascular diseases, arthritis, and cancer, making it a key biomarker for studying these diseases and developing potential therapeutic strategies.
This ELISA kit provides researchers with a reliable and efficient method for quantifying FSTL1 levels, ultimately advancing our understanding of its role in disease pathogenesis and improving patient outcomes. Unlock the potential of your research with the Human FSTL1 ELISA Kit from Assay Genie.
Product Name:
Human FSTL1 (Follistatin Like Protein 1) ELISA Kit
SKU:
HUES01441
Target:
Human FSTL1 (Follistatin Like Protein 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human FSTL1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FSTL1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FSTL1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human FSTL1. You can calculate the concentration of Human FSTL1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-105
96-109
92-105
Average (%)
97
102
99
1:4
Range (%)
92-105
87-101
90-104
Average (%)
98
92
95
1:8
Range (%)
92-104
86-97
83-95
Average (%)
98
91
88
1:16
Range (%)
93-107
86-102
83-97
Average (%)
98
93
89
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-103
98
EDTA plasma (n=5)
93-107
100
Cell culture media (n=5)
89-104
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.05
1.82
8.3
1.05
1.92
8.3
Standard deviation
0.06
0.08
0.25
0.07
0.1
0.38
C V (%)
5.71
4.4
3.01
6.67
5.21
4.58
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human FSTL1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human FSTL1 in samples. No significant cross-reactivity or interference between Human FSTL1 and analogues was observed.
