The Human FSH (Follicle Stimulating Hormone) CLIA Kit is specially designed for the precise measurement of FSH levels in human serum and plasma samples. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results for various research purposes.FSH is a key hormone crucial for the regulation of reproductive processes in both males and females, including the development of ovarian follicles in females and sperm production in males.
Abnormal levels of FSH have been associated with infertility, polycystic ovary syndrome, and other reproductive disorders, making this kit an invaluable tool for studying and diagnosing such conditions.With its user-friendly protocol and reliable performance, the Human FSH CLIA Kit from AssayGenie is an indispensable resource for researchers and clinicians investigating reproductive health and related disorders.
Product Name:
Human FSH (Follicle-Stimulating Hormone) CLIA Kit
SKU:
HUES00666
Target:
Human FSH (Follicle-Stimulating Hormone)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
0.19 mIU/mL
Detection range:
0.31-20 mIU/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human FSH. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FSH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FSH, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human FSH. You can calculate the concentration of Human FSH in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-106
89-103
91-107
Average (%)
101
97
97
1:4
Range (%)
88-99
85-96
88-102
Average (%)
93
91
94
1:8
Range (%)
93-105
88-104
98-110
Average (%)
99
96
103
1:16
Range (%)
88-99
101-117
91-106
Average (%)
94
109
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-107
100
EDTA plasma (n=5)
88-102
94
Cell culture media (n=5)
91-103
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.92
3.04
7.18
0.87
2.8
7.73
Standard deviation
0.08
0.35
0.74
0.08
0.22
0.57
C V (%)
8.7
11.51
10.31
9.2
7.86
7.37
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human FSH concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human FSH in samples. No significant cross-reactivity or interference between Human FSH and analogues was observed.
