The Human FPN (Ferroportin) ELISA Kit is specifically designed for the precise measurement of ferroportin levels in human samples such as serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit ensures accurate and consistent results, making it an excellent choice for various research applications.Ferroportin is a key protein involved in iron metabolism, responsible for transporting iron out of cells and into the bloodstream.
Dysregulation of ferroportin has been linked to conditions like iron overload disorders and anemia, highlighting its importance as a potential biomarker for studying these diseases and developing targeted therapies.With the Human FPN ELISA Kit, researchers can confidently investigate the role of ferroportin in various physiological and pathological processes, paving the way for deeper insights into iron homeostasis and related disorders.
Product Name:
Human FPN (Ferroportin) ELISA Kit
SKU:
HUES03226
Target:
Human FPN (Ferroportin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human FPN. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FPN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FPN, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human FPN. You can calculate the concentration of Human FPN in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-100
88-100
91-104
Average (%)
94
94
98
1:4
Range (%)
88-100
80-93
85-97
Average (%)
95
86
91
1:8
Range (%)
93-106
84-97
84-100
Average (%)
98
89
91
1:16
Range (%)
86-101
83-97
89-100
Average (%)
93
89
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-96
90
EDTA plasma (n=5)
93-104
99
Cell culture media (n=5)
90-102
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.92
2.31
7.33
0.98
2.22
6.66
Standard deviation
0.05
0.12
0.23
0.06
0.1
0.29
C V (%)
5.43
5.19
3.14
6.12
4.5
4.35
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human FPN concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human FPN in samples. No significant cross-reactivity or interference between Human FPN and analogues was observed.
