Human FNDC5 (Fibronectin type Ⅲ domain-containing protein 5) ELISA Kit
The Human FNDC5 (Fibronectin type III domain-containing protein 5) ELISA Kit is a state-of-the-art assay designed to quantitatively measure FNDC5 protein levels in human samples.
FNDC5, a key regulator of metabolism and energy expenditure, plays a crucial role in modulating physiological processes such as mitochondrial biogenesis and thermogenesis. Understanding the expression levels of FNDC5 can provide valuable insights into metabolic homeostasis, exercise-induced adaptations, and potential therapeutic targets for metabolic disorders. With exceptional sensitivity and specificity, the Human FNDC5 ELISA Kit enables researchers to accurately assess FNDC5 protein levels, offering a deeper understanding of its metabolic functions and potential implications in the pathophysiology of metabolic diseases. By providing precise measurements of FNDC5, this ELISA kit empowers researchers to explore the intricate mechanisms underlying energy metabolism, exercise physiology, and metabolic health. Manufactured to stringent quality control standards, the Human FNDC5 ELISA Kit delivers reliable and reproducible results, ensuring robust performance in metabolic research studies. Researchers investigating FNDC5-mediated metabolic pathways and metabolic disorders can leverage this user-friendly kit to advance their research and contribute to the knowledge of FNDC5-related metabolic processes.
Product Name:
Human FNDC5 (Fibronectin type Ⅲ domain-containing protein 5) ELISA Kit
SKU:
AEES00670
Target:
Human FNDC5 (Fibronectin type Ⅲ domain-containing protein 5)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human FNDC5. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FNDC5 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FNDC5, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human FNDC5. You can calculate the concentration of Human FNDC5 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-109
85-96
93-109
Average (%)
101
91
100
1:4
Range (%)
87-100
83-96
85-101
Average (%)
93
89
92
1:8
Range (%)
88-101
80-94
84-98
Average (%)
94
86
89
1:16
Range (%)
91-107
86-96
88-99
Average (%)
98
91
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-101
93
EDTA plasma (n=5)
90-102
96
Cell culture media (n=5)
87-98
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.51
0.87
4.57
0.51
0.86
4.33
Standard deviation
0.03
0.05
0.14
0.03
0.05
0.23
C V (%)
5.88
5.75
3.06
5.88
5.81
5.31
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human FNDC5 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human FNDC5 in samples. No significant cross-reactivity or interference between Human FNDC5 and analogues was observed.
