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Human FN3KRP Recombinant Protein (RPPB2536)

SKU:
RPPB2536
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q9HA64
Research Area:
Enzymes
€309
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Description

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Product Name:Human FN3KRP Recombinant Protein
Product Code:RPPB2536
Size:20µg
Species:Human
Target:FN3KRP
Synonyms:Ketosamine-3-kinase, Fructosamine-3-kinase-related protein, FN3K-RP, FN3K-related protein, FN3KRP, FN3KL.
Source:Escherichia Coli
Physical Appearance:Sterile filtered colorless solution.
Formulation:FN3KRP protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.4M urea and 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSMEELLRR ELGCSSVRAT GHSGGGCISQ GRSYDTDQGR VFVKVNPKAE ARRMFEGEMA SLTAILKTNT VKVPKPIKVL DAPGGGSVLV MEHMDMRHLS SHAAKLGAQL ADLHLDNKKL GEMRLKEAGT VGRGGGQEER PFVARFGFDV VTCCGYLPQVNDWQEDWVVF YARQRIQPQM DMVEKESGDR EALQLWSALQ LKIPDLFRDL EIIPALLHGD LWGGNVAEDS SGPVIFDPAS FYGHSEYELA IAGMFGGFSS SFYSAYHGKI PKAPGFEKRL QLYQLFHYLN HWNHFGSGYR GSSLNIMRNL VK

Ketosamine-3-kinase (FN3KRP) catalyzes the phosphorylation of psicosamines and ribulosamines compared to the adjacent gene which encodes a highly analogous enzyme, fructosamine-3-kinase that has different substrate specificity. The activity of both enzymes may cause deglycation of proteins to reinstate their function. A high concentration of glucose can result in non-enzymatic oxidation of proteins by reaction of glucose and lysine residues (glycation). The proteins altered in this way, are less active or functional.

FN3KRP Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 332 amino acids (1-309 a.a) and having a molecular mass of 36.8kDa.FN3KRP is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:FN3KRP: Phosphorylates psicosamines and ribulosamines, but not fructosamines, on the third carbon of the sugar moiety. Protein- bound psicosamine 3-phosphates and ribulosamine 3-phosphates are unstable and decompose under physiological conditions. Thus phosphorylation leads to deglycation. Belongs to the fructosamine kinase family.
UniProt Protein Details:

Protein type:EC 2.7.1.-; Kinase, other

Chromosomal Location of Human Ortholog: 17q25.3

Molecular Function:kinase activity

Biological Process: phosphorylation

NCBI Summary:A high concentration of glucose can result in non-enzymatic oxidation of proteins by reaction of glucose and lysine residues (glycation). Proteins modified in this way are less active or functional. This gene encodes an enzyme which catalyzes the phosphorylation of psicosamines and ribulosamines compared to the neighboring gene which encodes a highly similar enzyme, fructosamine-3-kinase, which has different substrate specificity. The activity of both enzymes may result in deglycation of proteins to restore their function. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2012]
UniProt Code:Q9HA64
NCBI GenInfo Identifier:47606765
NCBI Gene ID:79672
NCBI Accession:Q9HA64.2
UniProt Secondary Accession:Q9HA64,Q969F4, Q9H0U7,
UniProt Related Accession:Q9HA64
Molecular Weight:309
NCBI Full Name:Ketosamine-3-kinase
NCBI Synonym Full Names:fructosamine 3 kinase related protein
NCBI Official Symbol:FN3KRP��
NCBI Official Synonym Symbols:FN3KL��
NCBI Protein Information:ketosamine-3-kinase; FN3K-RP; FN3K-related protein; fructosamine-3-kinase-related protein
UniProt Protein Name:Ketosamine-3-kinase
UniProt Synonym Protein Names:Fructosamine-3-kinase-related protein; FN3K-RP; FN3K-related protein
Protein Family:Ketosamine-3-kinase
UniProt Gene Name:FN3KRP��
UniProt Entry Name:KT3K_HUMAN

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