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Human FMS Like Tyrosine Kinase 3 (Flt3) ELISA Kit

SKU:
HUDL01090
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P36888
Sensitivity:
0.108ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
CD135,STK1,FLK2,Fms-Related Tyrosine Kinase 3,Stem Cell Tyrosine Kinase 1,FL Cytokine Receptor,Fetal Liver Kinase-2
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Human FMS Like Tyrosine Kinase 3 (Flt3) ELISA Kit

The Human FMS-like tyrosine kinase 3 (FLT3) ELISA Kit is a reliable tool for detecting and quantifying FLT3 levels in human samples including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides accurate and reproducible results, making it suitable for a variety of research applications.FLT3 is a key protein involved in regulating cell growth, differentiation, and survival. Abnormalities in FLT3 expression and activity have been linked to various hematologic malignancies, including acute myeloid leukemia (AML) and certain types of lymphoma.

Understanding FLT3 levels can provide valuable insights into disease progression and potential treatment strategies.By using the Human FLT3 ELISA Kit, researchers can gain a deeper understanding of FLT3's role in disease pathogenesis and progression, ultimately paving the way for the development of targeted therapies for FLT3-associated disorders.

Product Name:Human FMS Like Tyrosine Kinase 3 (Flt3) ELISA Kit
Product Code:HUDL01090
Size:96 Assays
Target:Flt3
Synonyms:CD135, STK1, FLK2, Fms-Related Tyrosine Kinase 3, Stem Cell Tyrosine Kinase 1, FL Cytokine Receptor, Fetal Liver Kinase-2
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)82-10292
EDTA plasma (n=5)91-9894
Heparin plasma (n=5)86-10193
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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