The Human FMOD (Fibromodulin) CLIA Kit is specifically designed for the precise measurement of fibromodulin levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers superior sensitivity and specificity, guaranteeing accurate and consistent results for various research purposes.Fibromodulin is a key protein involved in extracellular matrix production and organization, playing a vital role in tissue development and maintenance. Dysregulation of fibromodulin has been implicated in various diseases, including arthritis, cardiovascular disorders, and cancer, highlighting its significance as a potential biomarker for disease progression and therapeutic interventions.
The Human FMOD (Fibromodulin) CLIA Kit is an invaluable tool for researchers investigating the role of fibromodulin in health and disease, providing reliable data for advancing our understanding of tissue homeostasis and designing targeted treatment strategies.
Product Name:
Human FMOD (Fibromodulin) CLIA Kit
SKU:
HUES01119
Target:
Human FMOD (Fibromodulin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human FMOD. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FMOD and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FMOD, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human FMOD. You can calculate the concentration of Human FMOD in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-111
93-107
98-111
Average (%)
106
99
104
1:4
Range (%)
95-113
84-97
95-111
Average (%)
103
91
103
1:8
Range (%)
96-113
84-97
94-106
Average (%)
104
91
101
1:16
Range (%)
85-100
93-108
100-118
Average (%)
92
101
109
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-99
93
EDTA plasma (n=5)
95-105
100
Cell culture media (n=5)
87-102
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
188.35
345.03
1674.25
174.75
347.48
1630.15
Standard deviation
19.55
24.5
145.83
21.9
29.12
176.55
C V (%)
10.38
7.1
8.71
12.53
8.38
10.83
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human FMOD concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human FMOD in samples. No significant cross-reactivity or interference between Human FMOD and analogues was observed.
