The Human Flotillin-2 (Flot2) ELISA Kit is specifically designed for the precise measurement of Flot2 levels in human biological samples such as serum, plasma, and cell culture supernatants. This kit is characterized by its exceptional sensitivity and specificity, which guarantee accurate and consistent results, thus making it an invaluable tool for a diverse array of research studies.Flotillin-2 is a key protein that plays a pivotal role in various cellular processes, including signal transduction, membrane trafficking, and cell adhesion.
Its dysregulation has been associated with several diseases, including cancer, cardiovascular disorders, and neurological conditions. As such, Flot2 serves as a critical biomarker for the investigation of these diseases and the exploration of potential therapeutic interventions.Overall, the Human Flotillin-2 (Flot2) ELISA Kit offers researchers a reliable and efficient method for quantifying Flot2 levels in human samples, thereby facilitating groundbreaking discoveries and advancements in the field of biomedical research.
Product Name:
Human Flotillin 2 (FLOT2) ELISA Kit
Product Code:
HUDL01089
Size:
96 Assays
Target:
FLOT2
Synonyms:
ECS1, ESA, ESA1, M17S1, Epidermal Surface Antigen 1, Membrane Component,Chromosome 17,Surface Marker 1, 35kD Protein Identified By Monoclonal Antibody ECS-1
Detection Method:
Sandwich
Range:
0.625-40ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
