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Human FLOT2 / Flotillin 2 ELISA Kit

SKU:
HUFI02469
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q14254
Sensitivity:
0.375ng/ml
Range:
0.625-40ng/ml
ELISA Type:
Sandwich
Synonyms:
FLOT2
Reactivity:
Human
Research Area:
Cell Biology
$719
Frequently bought together:

Description

Human FLOT2/Flotillin 2 ELISA Kit

The Human Flot2 (Flotillin-2) ELISA Kit is specifically designed for the accurate detection of Flotillin-2 levels in human serum, plasma, and cell culture supernatants. This advanced kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.Flotillin-2 is a key protein involved in membrane microdomain organization and cell signaling pathways. It plays a critical role in processes such as cell adhesion, migration, and intracellular trafficking.

Dysregulation of Flotillin-2 has been implicated in various diseases, including cancer, Alzheimer's, and cardiovascular disorders, making it a valuable target for further investigation and potential therapeutic interventions.Enhance your research with the Human Flot2 ELISA Kit and uncover new insights into the role of Flotillin-2 in health and disease. Order now from AssayGenie and take your research to the next level.

Product Name:Human FLOT2 / Flotillin 2 ELISA Kit
Product Code:HUFI02469
Size:96 Assays
Alias:FLOT2
Detection method:Sandwich ELISA, Double Antibody
Application:This immunoassay kit allows for the in vitro quantitative determination of Human FLOT2 concentrations in serum plasma and other biological fluids.
Sensitivity:0.375ng/ml
Range:0.625-40ng/ml
Storage:4°C for 6 months
Note:For Research Use Only
Recovery:Matrices listed below were spiked with certain level of Human FLOT2 and the recovery rates were calculated by comparing the measured value to the expected amount of Human FLOT2 in samples.
MatrixRecovery range(%)Average(%)
serum(n=5)85-9993
EDTA plasma(n=5)87-10295
UFH plasma(n=5)90-10399
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Human FLOT2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample1:21:41:8
serum(n=5)85-101%89-104%89-101%
EDTA plasma(n=5)87-95%82-93%86-98%
UFH plasma(n=5)83-100%82-98%84-98%
CV(%):Intra-Assay: CV<8%
Inter-Assay: CV<10%
ComponentQuantityStorage
ELISA Microplate (Dismountable)8×12 strips4°C for 6 months
Lyophilized Standard24°C/-20°C
Sample/Standard Dilution Buffer20ml4°C
Biotin-labeled Antibody(Concentrated)120ul4°C (Protect from light)
Antibody Dilution Buffer10ml4°C
HRP-Streptavidin Conjugate(SABC)120ul4°C (Protect from light)
SABC Dilution Buffer10ml4°C
TMB Substrate10ml4°C (Protect from light)
Stop Solution10ml4°C
Wash Buffer(25X)30ml4°C
Plate Sealer5-

Other materials and equipment required:

  • Microplate reader with 450 nm wavelength filter
  • Multichannel Pipette, Pipette, microcentrifuge tubes and disposable pipette tips
  • Incubator
  • Deionized or distilled water
  • Absorbent paper
  • Buffer resevoir
UniprotQ14254
UniProt Protein Function:FLOT2: May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles. May be involved in epidermal cell adhesion and epidermal structure and function. Heterooligomeric complex of flotillin-1 and flotillin-2 and caveolin-1 and caveolin-2. Interacts with ECM29. In skin, expressed in epidermis and epidermal appendages but not in dermis. Expressed in all layers of the epidermis except the basal layer. In hair follicles, expressed in the suprabasal layer but not the basal layer. Also expressed in melanoma and carcinoma cell lines, fibroblasts and foreskin melanocytes. Belongs to the band 7/mec-2 family. Flotillin subfamily.
UniProt Protein Details:Protein type:Cell adhesion

Chromosomal Location of Human Ortholog: 17q11-q12

Cellular Component: focal adhesion; membrane; endocytic vesicle; perinuclear region of cytoplasm; plasma membrane; caveola; flotillin complex; vesicle; endosome; acrosomal membrane

Molecular Function:ionotropic glutamate receptor binding; protein binding; protease binding

Biological Process: epidermis development; protein stabilization; cell adhesion

NCBI Summary:Caveolae are small domains on the inner cell membrane involved in vesicular trafficking and signal transduction. This gene encodes a caveolae-associated, integral membrane protein, which is thought to function in neuronal signaling. [provided by RefSeq, Jul 2008]
UniProt Code:Q14254
NCBI GenInfo Identifier:254763294
NCBI Gene ID:2319
NCBI Accession:Q14254.2
UniProt Related Accession:Q14254
Molecular Weight:~ 49kDa
NCBI Full Name:Flotillin-2
NCBI Synonym Full Names:flotillin 2
NCBI Official Symbol:FLOT2  
NCBI Official Synonym Symbols:ESA; ECS1; ESA1; ECS-1; M17S1  
NCBI Protein Information:flotillin-2
UniProt Protein Name:Flotillin-2
UniProt Synonym Protein Names:Epidermal surface antigen; ESA; Membrane component chromosome 17 surface marker 1
Protein Family:Flotillin
UniProt Gene Name:FLOT2  
UniProt Entry Name:FLOT2_HUMAN

*Note: Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.

Before adding to wells, equilibrate the SABC working solution and TMB substrate for at least 30 min at 37°C. When diluting samples and reagents, they must be mixed completely and evenly. It is recommended to plot a standard curve for each test.

StepProtocol
1.Set standard, test sample and control (zero) wells on the pre-coated plate respectively, and then, record their positions. It is recommended to measure each standard and sample in duplicate. Wash plate 2 times before adding standard, sample and control (zero) wells!
2.Aliquot 0.1ml standard solutions into the standard wells.
3.Add 0.1 ml of Sample / Standard dilution buffer into the control (zero) well.
4.Add 0.1 ml of properly diluted sample ( Human serum, plasma, tissue homogenates and other biological fluids.) into test sample wells.
5.Seal the plate with a cover and incubate at 37 °C for 90 min.
6.Remove the cover and discard the plate content, clap the plate on the absorbent filter papers or other absorbent material. Do NOT let the wells completely dry at any time. Wash plate X2.
7.Add 0.1 ml of Biotin- detection antibody working solution into the above wells (standard, test sample & zero wells). Add the solution at the bottom of each well without touching the side wall.
8.Seal the plate with a cover and incubate at 37°C for 60 min.
9.Remove the cover, and wash plate 3 times with Wash buffer. Let wash buffer rest in wells for 1 min between each wash.
10.Add 0.1 ml of SABC working solution into each well, cover the plate and incubate at 37°C for 30 min.
11.Remove the cover and wash plate 5 times with Wash buffer, and each time let the wash buffer stay in the wells for 1-2 min.
12.Add 90 µl of TMB substrate into each well, cover the plate and incubate at 37°C in dark within 10-20 min. (Note: This incubation time is for reference use only, the optimal time should be determined by end user.) And the shades of blue can be seen in the first 3-4 wells (with most concentrated standard solutions), the other wells show no obvious color.
13.Add 50 µl of Stop solution into each well and mix thoroughly. The color changes into yellow immediately.
14. Read the O.D. absorbance at 450 nm in a microplate reader immediately after adding the stop solution.

When carrying out an ELISA assay it is important to prepare your samples in order to achieve the best possible results. Below we have a list of procedures for the preparation of samples for different sample types.

Sample TypeProtocol
SerumIf using serum separator tubes, allow samples to clot for 30 minutes at room temperature. Centrifuge for 10 minutes at 1,000x g. Collect the serum fraction and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles.

If serum separator tubes are not being used, allow samples to clot overnight at 2-8°C. Centrifuge for 10 minutes at 1,000x g. Remove serum and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles.

PlasmaCollect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples at 4°C for 15 mins at 1000 × g within 30 mins of collection. Collect the plasma fraction and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles. Note: Over haemolysed samples are not suitable for use with this kit.
Urine & Cerebrospinal FluidCollect the urine (mid-stream) in a sterile container, centrifuge for 20 mins at 2000-3000 rpm. Remove supernatant and assay immediately. If any precipitation is detected, repeat the centrifugation step. A similar protocol can be used for cerebrospinal fluid.
Cell culture supernatantCollect the cell culture media by pipette, followed by centrifugation at 4°C for 20 mins at 1500 rpm. Collect the clear supernatant and assay immediately.
Cell lysatesSolubilize cells in lysis buffer and allow to sit on ice for 30 minutes. Centrifuge tubes at 14,000 x g for 5 minutes to remove insoluble material. Aliquot the supernatant into a new tube and discard the remaining whole cell extract. Quantify total protein concentration using a total protein assay. Assay immediately or aliquot and store at ≤ -20 °C.
Tissue homogenatesThe preparation of tissue homogenates will vary depending upon tissue type. Rinse tissue with 1X PBS to remove excess blood & homogenize in 20ml of 1X PBS (including protease inhibitors) and store overnight at ≤ -20°C. Two freeze-thaw cycles are required to break the cell membranes. To further disrupt the cell membranes you can sonicate the samples. Centrifuge homogenates for 5 mins at 5000xg. Remove the supernatant and assay immediately or aliquot and store at -20°C or -80°C.
Tissue lysatesRinse tissue with PBS, cut into 1-2 mm pieces, and homogenize with a tissue homogenizer in PBS. Add an equal volume of RIPA buffer containing protease inhibitors and lyse tissues at room temperature for 30 minutes with gentle agitation. Centrifuge to remove debris. Quantify total protein concentration using a total protein assay. Assay immediately or aliquot and store at ≤ -20 °C.
Breast MilkCollect milk samples and centrifuge at 10,000 x g for 60 min at 4°C. Aliquot the supernatant and assay. For long term use, store samples at -80°C. Minimize freeze/thaw cycles.

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