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Human Flavin Containing Monooxygenase 1 (FMO1) ELISA Kit

SKU:
HUDL01092
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q01740
Sensitivity:
0.115ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
Dimethylaniline oxidase 1,Fetal hepatic flavin-containing monooxygenase 1
Reactivity:
Human
Sample Type:
Tissue Homogenates And Other Biological Fluids
$779
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Description

system_update_altDatasheet

Human Flavin Containing Monooxygenase 1 (FMO1) ELISA Kit

The Human Flavin-Containing Monooxygenase 1 (FMO1) ELISA Kit is a tool specifically designed for the accurate measurement of FMO1 levels in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides reliable and reproducible results, making it a valuable asset for various research applications.FMO1 is an important enzyme involved in the metabolism of various drugs and toxins in the human body.

Dysregulation of FMO1 activity has been associated with drug toxicity and adverse drug reactions, making it a key biomarker for pharmacological research and drug development.By utilizing the Human FMO1 ELISA Kit, researchers can gain valuable insights into the role of FMO1 in drug metabolism and toxicology, ultimately aiding in the development of safer and more effective therapeutic interventions.

Product Name:Human Flavin Containing Monooxygenase 1 (FMO1) ELISA Kit
Product Code:HUDL01092
Size:96 Assays
Target:FMO1
Synonyms:Dimethylaniline oxidase 1, Fetal hepatic flavin-containing monooxygenase 1
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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