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Human FKBP4 Recombinant Protein (RPPB1644)

SKU:
RPPB1644
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q02790
Research Area:
Enzymes
€309
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Description

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Product Name:Human FKBP4 Recombinant Protein
Product Code:RPPB1644
Size:50µg
Species:Human
Target:FKBP4
Synonyms:HBI, p52, Hsp56, FKBP52, FKBP59, PPIase, FKBP4, FK506-binding protein 4, Peptidyl-prolyl cis-trans isomerase, HSP-binding immunophilin, FKBP52 protein, 52 kDa FK506-binding protein, p59 protein.
Source:Escherichia Coli
Physical Appearance:Sterile filtered colorless solution.
Formulation:The FKBP4 protein solution contains 20mM Tris-HCl, pH-8 and 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Purity:Greater than 90.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MTAEEMKATE SGAQSAPLPM EGVDISPKQD EGVLKVIKRE GTGTEMPMIG DRVFVHYTGW LLDGTKFDSS LDRKDKFSFDLGKGEVIKAW DIAIATMKVG EVCHITCKPE YAYGSAGSPP KIPPNATLVF EVELFEFKGE DLTEEEDGGI IRRIQTRGEG YAKPNEGAIV EVALEGYYKDKLFDQRELRF EIGEGENLDL PYGLERAIQR MEKGEHSIVY LKPSYAFGSV GKEKFQIPPN AELKYELHLK SFEKAKESWE MNSEEKLEQS TIVKERGTVYFKEGKYKQAL LQYKKIVSWL EYESSFSNEE AQKAQALRLA SHLNLAMCHL KLQAFSAAIE SCNKALELDS NNEKGLFRRG EAHLAVNDFE LARADFQKVLQLYPNNKAAK TQLAVCQQRI RRQLAREKKL YANMFERLAE EENKAKAEAS SGDHPTDTEM KEEQKSNTAG SQSQVETEA
Biological Activity:Specific activity is > 300 nmoles/min/mg, and is defined as the amount of enzyme that cleaves 1umole of suc-AAFP-pNA per minute at 25C in Tris-Hcl pH 8.0 using chymotrypsin.

FKBP4 is part of the immunophilin protein family, which takes part in immunoregulation and necessary cellular processes concerning protein folding and trafficking. FKBP4 is a cis-trans prolyl isomerase that connects to the immunosuppressants FK506 and rapamycin. FKBP4 has high structural and functional similarity to FKBP1A, though, FKBP4 does not have immunosuppressant activity when complexed with FK506. FKBP4 is known to connect with phytanoyl-CoA alpha-hydroxylase. FKBP4 associates with HSP90 & HSP70 thus takes part in the intracellular trafficking of hetero-oligomeric forms of the steroid hormone receptors. FKBP4 highly associates with adeno-associated virus type 2 vectors (AAV) resulting in a considerable increase in AAV-mediated transgene expression in human cell lines. FKBP4 is involved in the optimal use of AAV vectors in human gene therapy.

FKBP4 produced in E.Coli is a single,non-glycosylated polypeptide chain containing 479 amino acids (1-459 a.a.) and having a molecular mass of 53.9 kDa.FKBP4 is fused to a 20 amino acid His Tag at N-terminus and purified by proprietary chromatographic techniques.

UniProt Protein Function:FKBP4: Immunophilin protein with PPIase and co-chaperone activities. Component of unligated steroid receptors heterocomplexes through interaction with heat-shock protein 90 (HSP90). May play a role in the intracellular trafficking of heterooligomeric forms of steroid hormone receptors between cytoplasm and nuclear compartments. The isomerase activity controls neuronal growth cones via regulation of TRPC1 channel opening. Acts also as a regulator of microtubule dynamics by inhibiting MAPT/TAU ability to promote microtubule assembly.
UniProt Protein Details:

Protein type:EC 5.2.1.8; Chaperone; Isomerase

Chromosomal Location of Human Ortholog: 12p13.33

Cellular Component: cytoplasm; cytosol; endoplasmic reticulum membrane; nucleoplasm

Molecular Function:FK506 binding; heat shock protein binding; peptidyl-prolyl cis-trans isomerase activity; protein binding; protein binding, bridging

Biological Process: negative regulation of microtubule polymerization or depolymerization; protein folding

NCBI Summary:The protein encoded by this gene is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. This encoded protein is a cis-trans prolyl isomerase that binds to the immunosuppressants FK506 and rapamycin. It has high structural and functional similarity to FK506-binding protein 1A (FKBP1A), but unlike FKBP1A, this protein does not have immunosuppressant activity when complexed with FK506. It interacts with interferon regulatory factor-4 and plays an important role in immunoregulatory gene expression in B and T lymphocytes. This encoded protein is known to associate with phytanoyl-CoA alpha-hydroxylase. It can also associate with two heat shock proteins (hsp90 and hsp70) and thus may play a role in the intracellular trafficking of hetero-oligomeric forms of the steroid hormone receptors. This protein correlates strongly with adeno-associated virus type 2 vectors (AAV) resulting in a significant increase in AAV-mediated transgene expression in human cell lines. Thus this encoded protein is thought to have important implications for the optimal use of AAV vectors in human gene therapy. The human genome contains several non-transcribed pseudogenes similar to this gene. [provided by RefSeq, Sep 2008]
UniProt Code:Q02790
NCBI GenInfo Identifier:399866
NCBI Gene ID:2288
NCBI Accession:Q02790.3
UniProt Secondary Accession:Q02790,Q9UCP1, Q9UCV7, D3DUQ1,
UniProt Related Accession:Q02790
Molecular Weight:51,805 Da
NCBI Full Name:Peptidyl-prolyl cis-trans isomerase FKBP4
NCBI Synonym Full Names:FK506 binding protein 4
NCBI Official Symbol:FKBP4��
NCBI Official Synonym Symbols:HBI; p52; Hsp56; FKBP51; FKBP52; FKBP59; PPIase��
NCBI Protein Information:peptidyl-prolyl cis-trans isomerase FKBP4
UniProt Protein Name:Peptidyl-prolyl cis-trans isomerase FKBP4
UniProt Synonym Protein Names:51 kDa FK506-binding protein; FKBP51
Protein Family:Peptidyl-prolyl cis-trans isomerase
UniProt Gene Name:FKBP4��
UniProt Entry Name:FKBP4_HUMAN

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