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Human FK506 Binding Protein 4 (FKBP4) ELISA Kit

SKU:
HUDL01080
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q02790
Sensitivity:
0.057ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
FKBP52,FKBP59,HBI,Hsp56,p52,Rotamase,51 kDa FK506-binding protein,59 kDa immunophilin,HSP-binding immunophilin,Peptidyl-prolyl cis-trans isomerase FKBP4
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Human FK506 Binding Protein 4 (FKBP4) ELISA Kit

The Human FK506 binding protein 4 (FKBP4) ELISA Kit is a highly reliable and sensitive tool for the accurate detection of FKBP4 levels in human serum, plasma, and cell culture supernatants. This kit offers high specificity, ensuring consistent and accurate results for a variety of research applications.FKBP4 is a key protein that plays a crucial role in protein folding and cell signaling pathways. Dysregulation of FKBP4 has been linked to various diseases, including cancer, autoimmune disorders, and neurological conditions.

As such, FKBP4 serves as an important biomarker for studying these conditions and exploring potential therapeutic interventions.With its advanced technology and user-friendly design, the Human FKBP4 ELISA Kit from AssayGenie provides researchers with a powerful tool for advancing their scientific investigations and uncovering new insights into the role of FKBP4 in human health and disease.

Product Name:Human FK506 Binding Protein 4 (FKBP4) ELISA Kit
Product Code:HUDL01080
Size:96 Assays
Target:FKBP4
Synonyms:FKBP52, FKBP59, HBI, Hsp56, p52, Rotamase, 51 kDa FK506-binding protein, 59 kDa immunophilin, HSP-binding immunophilin, Peptidyl-prolyl cis-trans isomerase FKBP4
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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