ABP-278,AOI,FH1,FLN1L,LRS1,SCT,TABP,TAP,Actin Binding Protein 278,Actin-binding-like protein,Thyroid autoantigen,Filamin-3,Truncated actin-binding protein
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
The Human Filamin B Beta (FLNB) ELISA Kit is a reliable and sensitive tool for the quantification of Filamin B Beta levels in human serum, plasma, and cell culture supernatants. Filamin B Beta is a key protein involved in cytoskeletal organization and cell motility, making it crucial for various cellular processes.This ELISA kit offers high specificity and accuracy, ensuring precise and reproducible results for your research studies. Filamin B Beta dysfunction has been implicated in a variety of disorders, including musculoskeletal diseases, neurological disorders, and developmental abnormalities.
Therefore, measuring Filamin B Beta levels can provide valuable insights into these conditions and aid in the development of potential therapeutic strategies.Overall, the Human Filamin B Beta (FLNB) ELISA Kit is a valuable tool for researchers studying the role of Filamin B Beta in health and disease, providing a reliable means of quantifying this important protein in biological samples.
Product Name:
Human Filamin B Beta (FLNb) ELISA Kit
Product Code:
HUDL01087
Size:
96 Assays
Target:
FLNb
Synonyms:
ABP-278, AOI, FH1, FLN1L, LRS1, SCT, TABP, TAP, Actin Binding Protein 278, Actin-binding-like protein, Thyroid autoantigen, Filamin-3, Truncated actin-binding protein
Detection Method:
Sandwich
Range:
15.625-1000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
