The Human Fibulin-1 (FBLN1) ELISA Kit is specifically designed for the accurate quantification of Fibulin-1 levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.Fibulin-1, also known as FBLN1, is a crucial extracellular matrix protein that plays a vital role in regulating cell adhesion, migration, and tissue development. Abnormal levels of Fibulin-1 have been associated with various diseases, including cancer, cardiovascular disorders, and connective tissue disorders.
Therefore, this ELISA kit serves as a valuable tool for studying the role of Fibulin-1 in disease pathogenesis and for identifying potential therapeutic targets.With its reliable performance and ease of use, the Human Fibulin-1 ELISA Kit from Assay Genie is an indispensable resource for researchers seeking to understand the functional significance of Fibulin-1 in health and disease. Order yours today and unlock new insights into the molecular mechanisms underlying various pathological conditions.
Product Name:
Human Fibulin 1 (FBLN1) ELISA Kit
Product Code:
HUDL01027
Size:
96 Assays
Target:
FBLN1
Detection Method:
Sandwich
Range:
0.468-30ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
