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Human Fibronectin Type III Domain Containing Protein 5 (FNDC5) ELISA Kit

SKU:
HUDL01097
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q8NAU1
Sensitivity:
0.63ng/mL
Range:
1.56-100ng/mL
ELISA Type:
Sandwich
Synonyms:
FRCP2,Irisin,Fibronectin type III repeat-containing protein 2
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€749
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Description

system_update_altDatasheet

Human Fibronectin Type III Domain Containing Protein 5 (FNDC5) ELISA Kit

The Human FNDC5 ELISA Kit is specifically designed for the quantitative detection of human fibronectin type III domain-containing protein 5 (FNDC5) levels in serum, plasma, and cell culture supernatants. With a high level of sensitivity and specificity, this kit ensures reliable and reproducible results, making it an invaluable tool for research in a variety of fields.FNDC5 is a protein known for its role in regulating metabolism and promoting the production of irisin, a hormone that affects energy expenditure and has potential therapeutic benefits for conditions such as obesity and diabetes.

The measurement of FNDC5 levels can provide valuable insights into these metabolic processes and aid in the development of innovative treatment strategies.Whether studying metabolic disorders, exploring the mechanisms of exercise-induced changes in metabolism, or investigating the potential of irisin as a therapeutic target, the Human FNDC5 ELISA Kit provides researchers with a precise and efficient method for quantifying FNDC5 levels in biological samples.

Product Name:Human Fibronectin Type III Domain Containing Protein 5 (FNDC5) ELISA Kit
Product Code:HUDL01097
Size:96 Assays
Target:FNDC5
Synonyms:FRCP2, Irisin, Fibronectin type III repeat-containing protein 2
Detection Method:Sandwich
Range:1.56-100ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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