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Human Fibroblast Growth Factor Receptor 2 (FGFR2) ELISA Kit

SKU:
HUDL01069
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P21802
Sensitivity:
12.2pg/mL
Range:
31.25-2000pg/mL
ELISA Type:
Sandwich
Synonyms:
CD332,BEK,BFR1,CEK3,CFD1,ECT1,JWS,K-SAM,KGFR,TK14,Bacteria-Expressed Kinase,Keratinocyte Growth Factor Receptor,Craniofacial Dysostosis 1,Crouzon Syndrome
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
$719
Frequently bought together:

Description

system_update_altDatasheet

Human Fibroblast Growth Factor Receptor 2 (FGFR2) ELISA Kit

The Human Fibroblast Growth Factor Receptor 2 (FGFR2) ELISA Kit is a powerful tool for the precise measurement of FGFR2 levels in human samples including serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit delivers accurate and consistent results, making it indispensable for a variety of research endeavors.FGFR2 is a key receptor involved in cell growth and development, playing a critical role in various physiological processes.

Dysregulation of FGFR2 has been linked to multiple diseases, including cancer and skeletal disorders, highlighting its importance as a potential therapeutic target and diagnostic marker. By utilizing this ELISA kit, researchers can gain valuable insights into FGFR2 biology and advance their understanding of related pathologies.

Product Name:Human Fibroblast Growth Factor Receptor 2 (FGFR2) ELISA Kit
Product Code:HUDL01069
Size:96 Assays
Target:FGFR2
Synonyms:CD332, BEK, BFR1, CEK3, CFD1, ECT1, JWS, K-SAM, KGFR, TK14, Bacteria-Expressed Kinase,Keratinocyte Growth Factor Receptor, Craniofacial Dysostosis 1, Crouzon Syndrome
Detection Method:Sandwich
Range:31.25-2,000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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