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Human Fibroblast Growth Factor 3 (FGF3) ELISA Kit

SKU:
HUDL01061
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P11487
Sensitivity:
6.4pg/mL
Range:
15.625-1000pg/mL
ELISA Type:
Sandwich
Synonyms:
HBGF3,INT2,Heparin-binding growth factor 3,Murine Mammary Tumor Virus Integration Site(v-int-2)Oncogene Homolog,INT-2 Proto-Oncogene Protein,Proto-oncogene Int-2
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€599
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Description

system_update_altDatasheet

Human Fibroblast Growth Factor 3 (FGF3) ELISA Kit

The Human Fibroblast Growth Factor 3 (FGF3) ELISA Kit is a powerful tool for detecting and quantifying FGF3 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.FGF3 is a key growth factor that plays a vital role in cell growth, differentiation, and tissue repair. Dysregulation of FGF3 is associated with various diseases, including cancers and developmental disorders, making it an important biomarker for understanding these conditions and developing targeted therapies.

This ELISA kit provides researchers with a reliable method for studying the role of FGF3 in disease progression and therapeutic interventions. With its easy-to-use format and accurate results, the Human FGF3 ELISA Kit is an essential tool for investigating the function of FGF3 in health and disease.

Product Name:Human Fibroblast Growth Factor 3 (FGF3) ELISA Kit
Product Code:HUDL01061
Size:96 Assays
Target:FGF3
Synonyms:HBGF3, INT2, Heparin-binding growth factor 3, Murine Mammary Tumor Virus Integration Site(v-int-2)Oncogene Homolog, INT-2 Proto-Oncogene Protein, Proto-oncogene Int-2
Detection Method:Sandwich
Range:15.625-1,000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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