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Human Fibroblast Growth Factor 12 (FGF12) ELISA Kit

SKU:
HUDL01052
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P61328
Sensitivity:
8.1pg/mL
Range:
15.625-1000pg/mL
ELISA Type:
Sandwich
Synonyms:
FGF12B,FHF1,Fibroblast Growth Factor Homologous Factor 1,Myocyte-Activating Factor
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
$719
Frequently bought together:

Description

system_update_altDatasheet

Human Fibroblast Growth Factor 12 (FGF12) ELISA Kit

The Human Fibroblast Growth Factor 12 (FGF12) ELISA Kit is specifically designed for the precise detection of FGF12 levels in human serum, plasma, and cell culture supernatants. This innovative kit offers outstanding sensitivity and specificity, ensuring consistent and accurate results for a variety of research purposes.FGF12 is a vital protein that plays a critical role in cell growth and development, making it a key factor in various biological processes.

Its involvement in conditions such as cancer, cardiovascular diseases, and neurological disorders makes it a valuable biomarker for studying these diseases and exploring potential treatment options.With its advanced technology and reliable performance, the Human FGF12 ELISA Kit from AssayGenie is an essential tool for researchers looking to investigate the role of FGF12 in health and disease.

Product Name:Human Fibroblast Growth Factor 12 (FGF12) ELISA Kit
Product Code:HUDL01052
Size:96 Assays
Target:FGF12
Synonyms:FGF12B, FHF1, Fibroblast Growth Factor Homologous Factor 1, Myocyte-Activating Factor
Detection Method:Sandwich
Range:15.625-1,000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)90-10396
Heparin plasma (n=5)89-10295
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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