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Human Fibroblast Growth Factor 11 (FGF11) ELISA Kit

SKU:
HUDL01051
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q92914
Sensitivity:
5.9pg/mL
Range:
15.625-1000pg/mL
ELISA Type:
Sandwich
Synonyms:
FHF3,Fibroblast Growth Factor Homologous Factor 3
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
$719
Frequently bought together:

Description

system_update_altDatasheet

Human Fibroblast Growth Factor 11 (FGF11) ELISA Kit

The Human Fibroblast Growth Factor 11 (FGF11) ELISA Kit is a cutting-edge tool for the precise measurement of FGF11 levels in human samples including serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit delivers accurate and consistent results, making it an invaluable asset for various research endeavors.FGF11 is a key player in cell growth and differentiation processes, particularly in the development of the nervous system.

Its role in various diseases, including cancer and neurological disorders, underscores the significance of studying FGF11 levels as a potential biomarker for disease progression and therapeutic intervention. Equip yourself with the Human Fibroblast Growth Factor 11 (FGF11) ELISA Kit and unlock new insights into the intricate mechanisms governed by FGF11 in health and disease.

Product Name:Human Fibroblast Growth Factor 11 (FGF11) ELISA Kit
Product Code:HUDL01051
Size:96 Assays
Target:FGF11
Synonyms:FHF3, Fibroblast Growth Factor Homologous Factor 3
Detection Method:Sandwich
Range:15.625-1,000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)90-10396
Heparin plasma (n=5)89-10295
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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