null

Human Fibrinogen Like Protein 1 (FGL1) ELISA Kit

SKU:
HUDL01074
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q08830
Sensitivity:
0.055ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
FGL-1,HFREP1,HP-041,LFIRE1,Hepassocin,Hepatocyte-derived fibrinogen-related protein 1,Liver fibrinogen-related protein 1
Reactivity:
Human
Sample Type:
Plasma
€649
Frequently bought together:

Description

system_update_altDatasheet

Human Fibrinogen Like Protein 1 (FGL1) ELISA Kit

The Human FGL1 (Fibrinogen-Like Protein 1) ELISA Kit is specifically designed for the precise measurement of FGL1 levels in human samples such as serum, plasma, and cell culture supernatants. With outstanding sensitivity and specificity, this kit delivers accurate and consistent results, making it an excellent choice for various research purposes.FGL1 is a key protein involved in immune response regulation and is associated with various diseases, including cancer, liver disease, and inflammation.

Its detection and quantification can provide valuable insights into the pathogenesis of these conditions and help in the development of targeted therapies.Overall, the Human FGL1 ELISA Kit offers a reliable and efficient tool for researchers studying the role of FGL1 in health and disease, enabling them to advance their understanding and potentially uncover novel treatment options.

Product Name:Human Fibrinogen Like Protein 1 (FGL1) ELISA Kit
Product Code:HUDL01074
Size:96 Assays
Target:FGL1
Synonyms:FGL-1, HFREP1, HP-041, LFIRE1, Hepassocin, Hepatocyte-derived fibrinogen-related protein 1, Liver fibrinogen-related protein 1
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

Related Products