Human FHIT Recombinant Protein (RPPB3539)
- SKU:
- RPPB3539
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P49789
Description
| Product Name: | Human FHIT Recombinant Protein |
| Product Code: | RPPB3539 |
| Size: | 25µg |
| Species: | Human |
| Target: | FHIT |
| Synonyms: | EC 3.6.1.29, Dinucleosidetriphosphatase, Bis (5''-adenosyl)-triphosphatase , AP3Aase, AP3A hydrolase, Diadenosine 5'',5''''''-P1,P3-triphosphate hydrolase, FRA3B. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | FHIT Human solution containing 20mM Tris-HCl pH-8 & 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MSFRFGQHLI KPSVVFLKTE LSFALVNRKP VVPGHVLVCP LRPVERFHDL RPDEVADLFQ TTQRVGTVVE KHFHGTSLTF SMQDGPEAGQ TVKHVHVHVL PRKAGDFHRN DSIYEELQKH DKEDFPASWR SEEEMAAEAA ALRVYFQLEH HHHHH |
FHIT enzyme cleaves adenosine 5'' PPP 5'' A to yield AMP and ADP. FHIT gene includees the regular fragile site FRA3B on chromosome 3. Alterations and deletions of the FHIT gene are highly linked to the genesis and establishment of human tumors of the lung, cervix, breast, colon, stomach and pancreas. In normal cells, FHIT functions as a tumor suppressor and physically relates with ubiquitin conjugating enzyme 9.
FHIT Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 155 amino acids (1-147 a.a.) and having a molecular mass of 17.9 kDa. FHIT protein is fused to an 8 amino acid His tag at C-terminus and is purified by standard chromatography.
| UniProt Protein Function: | FHIT: a member of the histidine triad gene family. A diadenosine 5',5'''-P1,P3-triphosphate hydrolase involved in purine metabolism. Its gene encompasses the common fragile site FRA3B on chromosome 3, where carcinogen-induced damage can lead to translocations and aberrant transcripts of this gene. A possible tumor suppressor in specific tissues. Phospho-FHIT observed in liver and kidney, but not in brain and lung. Phospho-FHIT undetected in all tested human tumor cell lines. Aberrant transcripts from this gene have been found in about half of all esophageal, stomach, and colon carcinomas. |
| UniProt Protein Details: | Protein type:EC 3.6.1.29; Hydrolase; Motility/polarity/chemotaxis; DNA replication; Nucleotide Metabolism - purine; Tumor suppressor Chromosomal Location of Human Ortholog: 3p14.2 Cellular Component: cytoplasm; cytosol Molecular Function:bis(5'-adenosyl)-triphosphatase activity; catalytic activity; hydrolase activity; identical protein binding; protein binding; ubiquitin protein ligase binding Biological Process: negative regulation of proteasomal ubiquitin-dependent protein catabolic process; nucleotide metabolic process; purine nucleotide metabolic process |
| NCBI Summary: | This gene, a member of the histidine triad gene family, encodes a diadenosine 5',5'''-P1,P3-triphosphate hydrolase involved in purine metabolism. The gene encompasses the common fragile site FRA3B on chromosome 3, where carcinogen-induced damage can lead to translocations and aberrant transcripts of this gene. In fact, aberrant transcripts from this gene have been found in about half of all esophageal, stomach, and colon carcinomas. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Oct 2009] |
| UniProt Code: | P49789 |
| NCBI GenInfo Identifier: | 1706794 |
| NCBI Gene ID: | 2272 |
| NCBI Accession: | P49789.3 |
| UniProt Secondary Accession: | P49789,Q45QG9, Q6IU12, A2IAS9, A2IAT0, A2IAT6, A8K1A9 |
| UniProt Related Accession: | P49789 |
| Molecular Weight: | 16,858 Da |
| NCBI Full Name: | Bis(5'-adenosyl)-triphosphatase |
| NCBI Synonym Full Names: | fragile histidine triad |
| NCBI Official Symbol: | FHIT�� |
| NCBI Official Synonym Symbols: | FRA3B; AP3Aase�� |
| NCBI Protein Information: | bis(5'-adenosyl)-triphosphatase |
| UniProt Protein Name: | Bis(5'-adenosyl)-triphosphatase |
| UniProt Synonym Protein Names: | AP3A hydrolase; AP3Aase; Diadenosine 5',5'''-P1,P3-triphosphate hydrolase; Dinucleosidetriphosphatase; Fragile histidine triad protein |
| Protein Family: | Bis(5'-adenosyl)-triphosphatase |
| UniProt Gene Name: | FHIT�� |
| UniProt Entry Name: | FHIT_HUMAN |
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