The Human FGL2 (Fibrinogen-Like Protein 2) CLIA Kit is specifically designed for the accurate measurement of FGL2 levels in human samples such as serum, plasma, and cell culture supernatants. This CLIA kit offers high sensitivity and specificity, providing researchers with reliable and reproducible results for a variety of research applications.FGL2 is a critical protein involved in immune regulation and inflammation, playing a crucial role in conditions such as autoimmune diseases, organ transplantation, and viral infections.
By accurately measuring FGL2 levels, researchers can gain valuable insights into the pathogenesis of these conditions and potential therapeutic targets.Overall, the Human FGL2 CLIA Kit is a valuable tool for studying the role of FGL2 in various disease states and discovering new diagnostic and treatment strategies.
Product Name:
Human FGL2 (Fibrinogen Like Protein 2) CLIA Kit
SKU:
HUES01128
Target:
Human FGL2 (Fibrinogen Like Protein 2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human FGL2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FGL2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FGL2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human FGL2. You can calculate the concentration of Human FGL2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-100
100-117
90-107
Average (%)
94
108
98
1:4
Range (%)
94-109
96-111
90-104
Average (%)
100
104
97
1:8
Range (%)
90-103
100-118
95-108
Average (%)
95
108
101
1:16
Range (%)
88-99
99-115
100-114
Average (%)
94
107
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-106
99
EDTA plasma (n=5)
100-116
106
Cell culture media (n=5)
87-98
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
55.07
138.96
388.61
51.49
129.67
375.06
Standard deviation
5.82
12.09
29.65
4.65
14.61
40.54
C V (%)
10.57
8.7
7.63
9.03
11.27
10.81
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human FGL2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human FGL2 in samples. No significant cross-reactivity or interference between Human FGL2 and analogues was observed.
