The Human FGL1 (Fibrinogen-Like Protein 1) ELISA Kit is a cutting-edge tool for the accurate measurement of FGL1 levels in human biological samples such as serum, plasma, and cell culture supernatants. This kit boasts exceptional sensitivity and specificity, ensuring precise and consistent results for various research applications.FGL1 is a key protein that mimics the function of fibrinogen, playing a critical role in various physiological processes including liver regeneration, immune response modulation, and metabolic regulation.
Dysregulation of FGL1 expression has been implicated in liver diseases, inflammation, and cancer, making it a valuable biomarker for investigating these pathologies and developing targeted therapies.By utilizing the Human FGL1 ELISA Kit, researchers can delve deeper into the intricate mechanisms underlying FGL1's functions, paving the way for a better understanding of its role in health and disease. Trust in this advanced kit to elevate your research endeavors and unlock new insights into FGL1 biology.
Product Name:
Human FGL1 (Fibrinogen Like Protein 1) ELISA Kit
SKU:
HUES02666
Target:
Human FGL1 (Fibrinogen Like Protein 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human FGL1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FGL1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FGL1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human FGL1. You can calculate the concentration of Human FGL1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
84-98
99-113
88-103
Average (%)
90
105
94
1:4
Range (%)
94-109
86-97
86-102
Average (%)
100
92
93
1:8
Range (%)
90-107
82-94
83-97
Average (%)
97
86
90
1:16
Range (%)
90-105
83-95
82-93
Average (%)
96
89
87
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-108
98
EDTA plasma (n=5)
86-100
92
Cell culture media (n=5)
93-110
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.54
0.92
4.28
0.55
0.99
4.28
Standard deviation
0.03
0.04
0.15
0.03
0.05
0.2
C V (%)
5.56
4.35
3.5
5.45
5.05
4.67
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human FGL1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human FGL1 in samples. No significant cross-reactivity or interference between Human FGL1 and analogues was observed.
