Human FGFR4 (Fibroblast Growth Factor Receptor 4) ELISA Kit
The Human FGFR4 (Fibroblast Growth Factor Receptor 4) ELISA Kit is meticulously developed to provide quantitative analysis of FGFR4 levels in various human biological samples.
FGFR4, a member of the fibroblast growth factor receptor family, plays a crucial role in cell growth, differentiation, and tissue development. It is involved in various physiological processes, making it a significant receptor to study in the context of cell signaling pathways, oncogenesis, and developmental biology. Accurate measurement of FGFR4 levels is essential for understanding its diverse functions in health and disease. The FGFR4 ELISA Kit offered by Assay Genie stands out for its exceptional sensitivity, specificity, and reproducibility, ensuring precise quantification of this critical biomarker. Manufactured under stringent quality control standards, this kit ensures reliable performance and ease of use, making it an ideal choice for both research and clinical applications. Trust Assay Genie's FGFR4 ELISA Kit for accurate and dependable quantification of FGFR4 in your studies.
Product Name:
Human FGFR4 (Fibroblast Growth Factor Receptor 4) ELISA Kit
SKU:
AEES00071
Target:
Human FGFR4 (Fibroblast Growth Factor Receptor 4)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human FGFR4. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FGFR4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FGFR4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human FGFR4. You can calculate the concentration of Human FGFR4 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
83-97
96-108
89-101
Average (%)
91
100
97
1:4
Range (%)
101-111
92-103
95-102
Average (%)
105
99
98
1:8
Range (%)
90-103
97-108
90-97
Average (%)
95
103
93
1:16
Range (%)
93-107
99-108
92-102
Average (%)
101
104
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-108
100
EDTA plasma (n=5)
87-98
94
Cell culture media (n=5)
90-101
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
247.66
595.52
2334.42
245.13
539.87
2151.33
Standard deviation
13.6
29.3
105.52
14.36
24.94
107.57
C V (%)
5.49
4.92
4.52
5.86
4.62
5.0
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human FGFR4 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human FGFR4 in samples. No significant cross-reactivity or interference between Human FGFR4 and analogues was observed.
