Human FGFR4 (Fibroblast Growth Factor Receptor 4) CLIA Kit
Based on the information from the provided URL, here is a product description for the Human FGFR4 (Fibroblast Growth Factor Receptor 4) CLIA Kit:The Human FGFR4 (Fibroblast Growth Factor Receptor 4) CLIA Kit is specifically designed for the accurate detection of FGFR4 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.FGFR4 is a critical protein involved in cell growth and differentiation, playing a key role in various cellular processes.
Dysregulation of FGFR4 has been linked to diseases such as cancer, making it a valuable biomarker for studying disease progression and potential therapeutic interventions.With its robust performance and reliable results, the Human FGFR4 CLIA Kit is an essential tool for researchers and clinicians looking to better understand FGFR4 signaling pathways and its implications in health and disease. Visit the provided URL for more information on this innovative kit.
Product Name:
Human FGFR4 (Fibroblast Growth Factor Receptor 4) CLIA Kit
SKU:
HUES00254
Target:
Human FGFR4 (Fibroblast Growth Factor Receptor 4)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human FGFR4. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FGFR4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FGFR4, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human FGFR4. You can calculate the concentration of Human FGFR4 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-101
96-109
89-102
Average (%)
94
103
95
1:4
Range (%)
96-107
95-110
94-107
Average (%)
102
102
100
1:8
Range (%)
102-116
96-111
93-110
Average (%)
109
104
101
1:16
Range (%)
91-104
85-98
99-113
Average (%)
96
92
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-106
97
EDTA plasma (n=5)
95-109
103
Cell culture media (n=5)
83-98
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
96.5
310.09
682.92
104.11
299.51
627.13
Standard deviation
10.89
28.22
54.43
9.09
27.32
46.72
C V (%)
11.28
9.1
7.97
8.73
9.12
7.45
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human FGFR4 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human FGFR4 in samples. No significant cross-reactivity or interference between Human FGFR4 and analogues was observed.
